Mouse cyclin A Monoclonal Antibody | anti-CCNA2 antibody

cyclin A (E67.1) Antibody

Cat. Num. AAA440094

• Gene Names: CCNA2; CCN1; CCNA
• Reactivity: Cow, hamster, mink, mouse, human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Synonyms: cyclin A; Monoclonal Antibody; cyclin A (E67.1) Antibody; anti-CCNA2 antibody

• Host: Mouse
• Reactivity: Cow, hamster, mink, mouse, human
• Clonality: Monoclonal
• Specificity: Reacts with cyclin A of cow, hamster, mink, mouse, and human origin
• Form/Format: 100 ug/ml of mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
• Concentration: 0.100 mg/ml (varies by lot)
• Sequence Length: 432

• Applicable Applications for anti-CCNA2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western blotting starting dilution: 1:100.
• Origin: Cyclin A (E67.1) is provided as mouse monoclonal IgG2a derived from a mouse immunized with bovine cyclin A. The epitope for this antibody has been mapped to a region containing the sequence LDYP. This epitope maps within amino acids 144-148 of human cyclin A2.
• Immunogen: Bovine cyclin A
• Preparation and Storage: Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Related Product Information for anti-CCNA2 antibody

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

References

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63. 7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

NCBI and Uniprot Product Information

• NCBI GI #: 4502613
• NCBI GeneID: 890
• NCBI Accession #: NP_001228.1
• NCBI GenBank Nucleotide #: NM_001237.3
• UniProt Accession #: P20248; Q2M3U6; Q4W5P4; Q6LER8; A8K7B6
• Molecular Weight: 48,551 Da
• NCBI Official Full Name: cyclin-A2
• NCBI Official Synonym Full Names: cyclin A2
• NCBI Official Symbol: CCNA2
• NCBI Official Synonym Symbols: CCN1; CCNA
• NCBI Protein Information: cyclin-A2
• UniProt Protein Name: Cyclin-A2
• Protein Family: Cyclin
• UniProt Gene Name: CCNA2
• UniProt Synonym Gene Names: CCN1; CCNA; Cyclin-A
• UniProt Entry Name: CCNA2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008]

Uniprot Description

CCNA2: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 4q27

Cellular Component: nucleoplasm; male pronucleus; cytoplasm; female pronucleus; nucleus

Molecular Function: protein binding; protein kinase binding

Biological Process: mitosis; organ regeneration; response to glucagon stimulus; positive regulation of transcription, DNA-dependent; response to estradiol stimulus; positive regulation of fibroblast proliferation; cell division; mitotic cell cycle G2/M transition DNA damage checkpoint; regulation of G2/M transition of mitotic cell cycle; Ras protein signal transduction; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; G2/M transition of mitotic cell cycle