Rabbit Cullin 1/CUL-1 Monoclonal Antibody | anti-CUL1 antibody

Cullin 1/CUL-1 Rabbit mAb

Cat. Num. AAA4752674

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Affinity purified
• Synonyms: Cullin 1/CUL-1; Monoclonal Antibody; Cullin 1/CUL-1 Rabbit mAb; Cullin-1; anti-CUL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R05-3D2
• Specificity: Expressed in lung fibroblasts.
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-CUL1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; ICC/IF: 1:20-1:50; FC/FACS: 1:20
• Immunogen: A synthetic peptide of human Cullin 1
• Conjugation: Unconjugated
• Modification: Unmodification
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of Cullin 1/CUL-1 in K562,C6 cell lysates using Cullin 1/CUL-1 Rabbit mAb(1:1000 diluted). Predicted band size:90kDa. Observed band size:90kDa.)

Related Product Information for anti-CUL1 antibody

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(BTRC) and/or SCF(FBXW11) direct ubiquitination of CEP68 (PubMed:25704143, PubMed:25503564). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of CCNE1, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO1) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.

NCBI and Uniprot Product Information

• NCBI GI #: 32307161
• NCBI GeneID: 8454
• NCBI Accession #: NP_003583.2
• NCBI GenBank Nucleotide #: NM_003592.2
• UniProt Accession #: Q13616; O60719; Q08AL6; Q8IYW1; D3DWG3
• Molecular Weight: 89,679 Da
• NCBI Official Full Name: cullin-1
• NCBI Official Synonym Full Names: cullin 1
• NCBI Official Symbol: CUL1
• NCBI Protein Information: cullin-1; CUL-1
• UniProt Protein Name: Cullin-1
• Protein Family: Cullin
• UniProt Gene Name: CUL1
• UniProt Synonym Gene Names: CUL-1
• UniProt Entry Name: CUL1_HUMAN

Uniprot Description

CUL1: a core scaffolding component of multiple cullin-RING-based SCF (SKP1- CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. The cullin subunit serves as a rigid scaffold of SCF complexes. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex depends on the neddylation of the cullin subunit. Interacts with multiple F-box proteins in SCF complexes, directing the ubiquitination of many substrates. These F-box proteins and associated substrates include: BTRC and FBXW11 bind CTNNB1 and participate in Wnt signaling; FBXW11 bind phosphorylated NFKBIA; BTRC binds NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2; SKP2 binds phosphorylated p27kip and is involved in regulation of G1/S transition; SKP2 binds ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1; FBXW7 binds cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1; FBXW2 binds GCM1; FBXO32 binds MYOD1; FBXO7 binds BIRC2 and DLGAP5; FBXO33 binds YBX1; and Cyclin F binds CP110. Interacts with CHEK2, mediating its ubiquitination and regulating its function. Belongs to the cullin family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; cullin-RING ubiquitin ligase complex; SCF ubiquitin ligase complex; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; circadian rhythm; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; viral reproduction; protein ubiquitination; negative regulation of cell proliferation; cell proliferation; organ morphogenesis; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; cell cycle arrest; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle