Mouse anti-Bovine Crystallin, beta Monoclonal Antibody

Crystallin, beta

Cat. Num. AAA608345

• Reactivity: Bovine
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Crystallin; beta; Monoclonal Antibody; Anti -Crystallin; anti-Crystallin; beta antibody

• Host: Mouse
• Reactivity: Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4E56
• Specificity: Detects a ~25kD protein, corresponding to the apparent molecular mass of beta-crystallin on SDS-PAGE immunoblots, in samples from bovine. There may be weak reactivity with alphaA crystallin detected on immunoblot analysis.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF in 50% glycerol.

• Applicable Applications for anti-Crystallin, beta antibody: Western Blot (WB)
• Immunogen: Bovine betaH-crystallin
• Positive Control: Bovine beta-Crystallin Protein
• Preparation and Storage: -20 degree C

Related Product Information for anti-Crystallin, beta antibody

The beta-crystallins comprise a complex group of heteropolymers which are assembled from 6 primary gene products, the acidic (betaA1, betaA2, betaA3) and the basic (betaB1, betaB2, betaB3) polypeptides. betaA1 and betaA3 are identical, except for their N-termini, and appear to be generated by alternative translation of the same gene. betaB1 occurs in two forms, the primary gene product, betaB1a, and betaB1b which is generated through proteolysis. Further heterogeneity stems from post-translational modifications such as phosphorylation and/or other modifications that accumulate with aging. The subunits range in size from 23-25 kD for the betaA and 26-32kD for the betaB polypeptides. The beta-crystallins can be isolated in several aggregation states, spanning a molecular weight range from about 46kD to > 200kD. These include the octameric betaH, the tetrameric betaL1, and the dimeric betaL2 forms. beta-crystallins generally represent the second most abundant group of proteins in the lens but their proportions and properties vary with development. In the prenatal bovine lens, beta-crystallins account for 30% of the total proteins and betaH is predominant. In the adult, the total has increased to 40% due to an increased production of the betaL species. With the exception of betaB1a and betaB1b which are found only in betaH and appear to be responsible for its aggregation, all polypeptides are found in all forms of the protein. The betaB2 polypeptide (previously called betaBp) predominates, accounting for about 50% of the total. The lack of stoichiometry in the subunit contents suggests that each of the different molecular weight populations is a mixture of aggregates with most of the major polypeptides represented. These various species appear to be in equilibrium and their proportions vary with protein concentration. It is likely that even more highly aggregated forms, are the major species in the lens (2-13).

Product Categories/Family for anti-Crystallin, beta antibody

Antibodies; Abs to Crystallins

NCBI and Uniprot Product Information

• NCBI GI #: 557548
• NCBI Official Full Name: crystallin, partial

Uniprot Description

CRYGS: Crystallins are the dominant structural components of the vertebrate eye lens. Belongs to the beta/gamma-crystallin family.

Chromosomal Location of Human Ortholog: 3q27.3

Molecular Function: structural constituent of eye lens

Biological Process: lens development in camera-type eye; morphogenesis of an epithelium

Disease: Cataract 20, Multiple Types