Mouse anti-Human CRTAM Monoclonal Antibody | anti-CRTAM antibody

Anti-CRTAM Antibody, Mouse Monoclonal

Cat. Num. AAA8106043

• Gene Names: CRTAM; CD355
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: CRTAM; Monoclonal Antibody; Anti-CRTAM Antibody; Mouse Monoclonal; CRTAM Antibody; Mouse MAb; cytotoxic and regulatory T cell molecule; Anti-CD355 Antibody; anti-CRTAM antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 06
• Specificity: Human CRTAM
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CRTAM antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human CRTAM Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CRTAM (rh CRTAM). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CRTAM antibody

Cytotoxic and regulatory T-cell molecule, also known as Class-I MHC-restricted T-cell-associated molecule and CRTAM, is a single-pass type I membrane protein which belongs to thenectin family.CRTAM contains oneIg-like C2-type (immunoglobulin-like) domain and oneIg-like V-type (immunoglobulin-like) domain. In the immune system, the expression of CRTAM is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. It is strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in purkinje neurons in cerebellum. It is expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. CRTAM is a member of the immunoglobulin superfamily that complies with the structural characteristics of the JAM family of proteins and is phylogenetically more closely related to nectin-like proteins. It is a molecule involved in epithelial cell adhesion. CRTAM is sensitive to intermediate filament disruption and treatment of monolayers with soluble CRTAM enhances cell-cell dissociation and lowers transepithelial electrical resistance. CRTAM may also induce retention by binding to CD8+ dendritic cells (DCs) at the late stage of activation before proliferation.

References

Garay, E. et al., 2010, J Cell Biochem. 111 (1):111-22. Medina-C.O. et al., 2010, Dev Comp Immunol. 34 (2):196-202. Takeuchi, A. et al., 2009, J Immunol.183 (7):4220-8. Valle-Rios, R. et al., 2009, Mol Immunol. 46 (16): 3379-87.

NCBI and Uniprot Product Information

• NCBI GI #: 51593098
• NCBI GeneID: 56253
• NCBI Accession #: NP_062550.2
• NCBI GenBank Nucleotide #: NM_019604.2
• UniProt Accession #: O95727; Q59EI1; Q6IRX2
• Molecular Weight: 44,641 Da
• NCBI Official Full Name: cytotoxic and regulatory T-cell molecule
• NCBI Official Synonym Full Names: cytotoxic and regulatory T cell molecule
• NCBI Official Symbol: CRTAM
• NCBI Official Synonym Symbols: CD355
• NCBI Protein Information: cytotoxic and regulatory T-cell molecule; class I MHC restricted T cell associated molecule; class-I MHC-restricted T cell associated molecule; class-I MHC-restricted T-cell-associated molecule
• UniProt Protein Name: Cytotoxic and regulatory T-cell molecule
• Protein Family: Cytotoxic and regulatory T-cell molecule
• UniProt Gene Name: CRTAM
• UniProt Entry Name: CRTAM_HUMAN

NCBI Description

The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).[supplied by OMIM, Feb 2009]

Uniprot Description

CRTAM: Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q24.1

Cellular Component: cell-cell adherens junction; integral to plasma membrane; plasma membrane

Molecular Function: cell adhesion molecule binding; protein homodimerization activity; receptor activity; receptor binding

Biological Process: activated T cell proliferation; cell recognition; detection of stimulus; detection of tumor cell; heterophilic cell adhesion; homophilic cell adhesion; positive regulation of cytokine secretion; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; regulation of immune response; T cell mediated cytotoxicity