Monkey Fibrillin 1 ELISA Kit | FBN1 elisa kit

Monkey Fibrillin 1 ELISA Kit

Cat. Num. AAA741896

• Gene Names: FBN1; FBN; SGS; WMS; MASS; MFS1; OCTD; SSKS; WMS2; ACMICD; ECTOL1; GPHYSD2
• Reactivity: Monkey
• Synonyms: Fibrillin 1; Monkey Fibrillin 1 ELISA Kit; FBN1 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This FBN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey FBN1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBN1 elisa kit

Principle of the Assay: FBN1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FBN1. Standards or samples are then added to the microtiter plate wells and FBN1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FBN1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for FBN1 are added to each well to "sandwich" the FBN1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FBN1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FBN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FBN1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 281485550
• NCBI GeneID: 2200
• NCBI Accession #: NP_000129.3
• NCBI GenBank Nucleotide #: NM_000138.4
• Molecular Weight: 312,237 Da
• NCBI Official Full Name: fibrillin-1
• NCBI Official Synonym Full Names: fibrillin 1
• NCBI Official Symbol: FBN1
• NCBI Official Synonym Symbols: FBN; SGS; WMS; MASS; MFS1; OCTD; SSKS; WMS2; ACMICD; ECTOL1; GPHYSD2
• NCBI Protein Information: fibrillin-1; fibrillin 15
• UniProt Protein Name: Fibrillin-1
• Protein Family: Fibrillin
• UniProt Gene Name: FBN1
• UniProt Synonym Gene Names: FBN
• UniProt Entry Name: FBN1_HUMAN

NCBI Description

This gene encodes a member of the fibrillin family. The encoded protein is a large, extracellular matrix glycoprotein that serve as a structural component of 10-12 nm calcium-binding microfibrils. These microfibrils provide force bearing structural support in elastic and nonelastic connective tissue throughout the body. Mutations in this gene are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

FBN1: a large, extracellular matrix glycoprotein of the fibrillin family that serves as a structural component of 10-12 nm calcium-binding microfibrils, which occur either in association with elastin or in elastin-free bundles. Fibrillin-1- containing microfibrils provide long-term force bearing structural support in elastic and nonelastic connective tissue throughout the body. May regulate osteoblast maturation by controlling TGF-beta bioavailability and calibrating TGF-beta and BMP levels, respectively. Defects in this protein are associated with Marfan syndrome, isolated ectopia lentis, autosomal dominant Weill-Marchesani syndrome, MASS syndrome, and Shprintzen-Goldberg craniosynostosis syndrome. Interacts with COL16A1.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; microfibril; basement membrane

Molecular Function: protein binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; heart development; metanephros development; skeletal development

Disease: Ectopia Lentis 1, Isolated, Autosomal Dominant; Mass Syndrome; Weill-marchesani Syndrome 2; Stiff Skin Syndrome; Acromicric Dysplasia; Marfan Syndrome; Geleophysic Dysplasia 2