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Western Blot (WB) (Western Blot analysis of COX6B1 expression in transfected 293T cell line by COX6B1 monoclonal antibody (M02), clone 2D3.Lane 1: COX6B1 transfected lysate (10.2 KDa).Lane 2: Non-transfected lysate.)

Mouse COX6B1 Monoclonal Antibody | anti-COX6B1 antibody

COX6B1 (Cytochrome c Oxidase Subunit Vib Polypeptide 1 (ubiquitous), COX6B, COXG) (HRP)

Gene Names
COX6B1; COXG; COX6B; COXVIb1
Applications
Western Blot
Purity
Purified
Synonyms
COX6B1; Monoclonal Antibody; COX6B1 (Cytochrome c Oxidase Subunit Vib Polypeptide 1 (ubiquitous); COX6B; COXG) (HRP); Cytochrome c Oxidase Subunit Vib Polypeptide 1 (ubiquitous); COXG; anti-COX6B1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2D3
Specificity
Recognizes COX6B1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
86
Applicable Applications for anti-COX6B1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
COX6B1 (AAH01015, 1aa-86aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MAEDMETKIKNYKTAPFDSRFPNQNQTRNCWQNYLDFHRCQKAMTAKGGDISVCEWYQRVYQSLCPTSWVTDWDEQRAEGTFPGKI
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of COX6B1 expression in transfected 293T cell line by COX6B1 monoclonal antibody (M02), clone 2D3.Lane 1: COX6B1 transfected lysate (10.2 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of COX6B1 expression in transfected 293T cell line by COX6B1 monoclonal antibody (M02), clone 2D3.Lane 1: COX6B1 transfected lysate (10.2 KDa).Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged COX6B1 is approximately 1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged COX6B1 is approximately 1ng/ml as a capture antibody.)
Product Categories/Family for anti-COX6B1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Cytochrome c oxidase subunit Vib polypeptide 1 (ubiquitous)
NCBI Official Synonym Full Names
cytochrome c oxidase subunit 6B1
NCBI Official Symbol
COX6B1
NCBI Official Synonym Symbols
COXG; COX6B; COXVIb1
NCBI Protein Information
cytochrome c oxidase subunit 6B1
Protein Family

NCBI Description

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Mutations in this gene are associated with severe infantile encephalomyopathy. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq, Jan 2010]

Research Articles on COX6B1

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Product Notes

The COX6B1 (Catalog #AAA6182212) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COX6B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the COX6B1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX6B1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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