Rabbit anti-Human CPNE8 Polyclonal Antibody | anti-CPNE8 antibody

CPNE8, NT (CPNE8, Copine-8, Copine VIII) (APC)

Cat. Num. AAA6288363

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPNE8; Polyclonal Antibody; NT (CPNE8; Copine-8; Copine VIII) (APC); Copine VIII; anti-CPNE8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CPNE8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: CPNE8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-111 amino acids from the N-terminal region of human CPNE8.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(CPNE8 Antibody (N-term) western blot analysis in 293,A375 cell line lysates (35ug/lane).This demonstrates the CPNE8 antibody detected the CPNE8 protein (arrow).)

Immunohistochemistry (IHC)

(CPNE8 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CPNE8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-CPNE8 antibody

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This protein is one of several genes that encode a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus.

Product Categories/Family for anti-CPNE8 antibody

Antibodies; Proteins

References

Daly, A.K., et al. Nat. Genet. 41(7):816-819(2009). Matsuoka, S., et al. Science 316(5828):1160-1166(2007). Ramsey, H., et al. Leukemia 17(8):1665-1666(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 25121972
• NCBI GeneID: 144402
• NCBI Accession #: NP_705898.1
• NCBI GenBank Nucleotide #: NM_153634.2
• UniProt Accession #: Q86YQ8; Q2TB41
• Molecular Weight: 63,108 Da
• NCBI Official Full Name: copine-8
• NCBI Official Synonym Full Names: copine VIII
• NCBI Official Symbol: CPNE8
• NCBI Protein Information: copine-8
• UniProt Protein Name: Copine-8
• Protein Family: Copine
• UniProt Gene Name: CPNE8
• UniProt Entry Name: CPNE8_HUMAN

NCBI Description

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encode a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. [provided by RefSeq, Jul 2008]

Uniprot Description

CPNE8: a member of the copine protein family with calcium-dependent phospholipid binding properties. Copines are ubiquitously expressed, phospholipid-binding proteins that have been conserved through evolution. Highest expression of CPNE8 mRNA is seen in the prostate, heart, and brain. Fusion to AML1 in a t(12;21) translocation causes aggressive acute myelogenous leukemia. Two alternatively spliced isoforms have been described.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 12q12