Western Blot (WB)
(Anti-COX5B rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa)
Rabbit anti-Human COX5B Monoclonal Antibody | anti-COX5B antibody
Recombinant Anti-COX5B Antibody, Rabbit Monoclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Western Blot (WB)
(Anti-COX5B rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa)
Western Blot (WB)
(Anti-COX5B rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:14 kDaObserved band size:15 kDa)
Immunoprecipitation (IP)
(COX5B was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate2 uL anti-COX5B rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX5B rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa)
Immunoprecipitation (IP)
(COX5B was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate2 uL anti-COX5B rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-COX5B rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 14 kDaObserved band size: 14 kDa)
NCBI and Uniprot Product Information
NCBI Description
Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]
Uniprot Description
COX5B: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5B family.
Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial
Chromosomal Location of Human Ortholog: 2q11.2
Cellular Component: mitochondrion; mitochondrial inner membrane
Molecular Function: cytochrome-c oxidase activity; metal ion binding
Biological Process: cellular metabolic process; respiratory gaseous exchange; transmembrane transport
Research Articles on COX5B
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Product Notes
The COX5B cox5b (Catalog #AAA8107630) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-COX5B Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COX5B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the COX5B cox5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COX5B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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