Western Blot (WB)
(The Recombinant Human TBCEL (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCEL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human TBCEL Lane 2.: PC3 cell lysate Lane 3.: LNCap cell lysate Lane 4 : 293T cell lysate Lane 5.: Mouse testis lysate Lane 6.: Mouse Kidney lysate)
Mouse anti-Human TBCEL Monoclonal Antibody | anti-TBCEL antibody
TBCEL antibody
Gene Names
TBCEL; El; LRRC35
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
TBCEL; Monoclonal Antibody; TBCEL antibody; Tubulin-specific chaperone cofactor E-like protein; El; LRRC35; anti-TBCEL antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1B10
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
424
Applicable Applications for anti-TBCEL antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Antigen Species
Human
Immunogen
Recombinant human TBCEL (1-424aa) purified from E. coli
Clone Note
Anti-human TBCEL mAb, clone AT1B10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TBCEL protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The Recombinant Human TBCEL (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCEL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human TBCEL Lane 2.: PC3 cell lysate Lane 3.: LNCap cell lysate Lane 4 : 293T cell lysate Lane 5.: Mouse testis lysate Lane 6.: Mouse Kidney lysate)
Western Blot (WB)
(The Recombinant Human TBCEL (50ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TBCEL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human TBCEL Lane 2.: PC3 cell lysate Lane 3.: LNCap cell lysate Lane 4 : 293T cell lysate Lane 5.: Mouse testis lysate Lane 6.: Mouse Kidney lysate)
Immunofluorescence (IF)
(ICC/IF analysis of TBCEL in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCEL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of TBCEL in PC3 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TBCEL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-TBCEL antibody
Tubulin-specific chaperone cofactor E-like protein, also known as TBCEL, is a factor that regulates the microtubule cytoskeleton in determining cell behavior.
Product Categories/Family for anti-TBCEL antibody
References
Bartolini F., et al. (2005) J Biomed Sci. 118: 1197-1207.; Ota T., et al. (2004) Nat Genet. 36: 40-45.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,195 Da
NCBI Official Full Name
tubulin-specific chaperone cofactor E-like protein
NCBI Official Synonym Full Names
tubulin folding cofactor E like
NCBI Official Symbol
TBCEL
NCBI Official Synonym Symbols
El; LRRC35
NCBI Protein Information
tubulin-specific chaperone cofactor E-like protein
UniProt Protein Name
Tubulin-specific chaperone cofactor E-like protein
Protein Family
UniProt Gene Name
TBCEL
UniProt Synonym Gene Names
LRRC35; EL
Uniprot Description
Acts as a regulator of tubulin stability.
Research Articles on TBCEL
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Product Notes
The TBCEL tbcel (Catalog #AAA200346) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TBCEL antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBCEL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the TBCEL tbcel for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBCEL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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