Mouse anti-Human COQ3 Monoclonal Antibody | anti-COQ3 antibody

COQ3 (Dihydroxyhexaprenylbenzoate Methyltransferase, 3,4-dihydroxy-5-hexaprenylbenzoate Methyltransferase, Hexaprenyldihydroxybenzoate Methyltransferase, Mitochondrial, DHHB Methyltransferasem, DHHB-MTase, DHHB-MT, COQ3, UG0215E05) (AP)

Cat. Num. AAA6130710

• Gene Names: COQ3; DHHBMT; bA9819.1; DHHBMTASE; UG0215E05
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COQ3; Monoclonal Antibody; COQ3 (Dihydroxyhexaprenylbenzoate Methyltransferase; 3; 4-dihydroxy-5-hexaprenylbenzoate Methyltransferase; Hexaprenyldihydroxybenzoate Methyltransferase; Mitochondrial; DHHB Methyltransferasem; DHHB-MTase; DHHB-MT; UG0215E05) (AP); anti-COQ3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6D5
• Specificity: Recognizes human COQ3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-COQ3 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa273-370 from human COQ3 (NP_059117) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: IVPKGTHTWEKFVSPETLESILESNGLSVQTVVGMLYNPFSGYWHWSENTSLNYAAHAVKSRVQEHPASAEFVLKGETEELQANACTNPAVHEKLKK
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (36.78kD).)

Western Blot (WB)

(Western Blot analysis of COQ3 expression in transfected 293T cell line by COQ3 monoclonal antibody. Lane 1: COQ3 transfected lysate (41kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-COQ3 antibody

Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. COQ3 is an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.

Product Categories/Family for anti-COQ3 antibody

Antibodies; Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 118600965
• NCBI GeneID: 51805
• NCBI Accession #: NP_059117
• NCBI GenBank Nucleotide #: NM_017421
• UniProt Accession #: Q9NZJ6
• Molecular Weight: 40kDa
• NCBI Official Full Name: ubiquinone biosynthesis O-methyltransferase, mitochondrial
• NCBI Official Synonym Full Names: coenzyme Q3, methyltransferase
• NCBI Official Symbol: COQ3
• NCBI Official Synonym Symbols: DHHBMT; bA9819.1; DHHBMTASE; UG0215E05
• NCBI Protein Information: ubiquinone biosynthesis O-methyltransferase, mitochondrial
• UniProt Protein Name: Hexaprenyldihydroxybenzoate methyltransferase, mitochondrial
• UniProt Gene Name: COQ3
• UniProt Synonym Gene Names: DHHB methyltransferase; DHHB-MT; DHHB-MTase
• UniProt Entry Name: COQ3_HUMAN

NCBI Description

Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM, Mar 2008]

Uniprot Description

Catalytic activity: S-adenosyl-L-methionine + 3,4-dihydroxy-5-all-trans-polyprenylbenzoate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate. Ref.6S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n. Ref.6S-adenosyl-L-methionine + 3-(all-trans-polyprenyl)benzene-1,2-diol = S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-polyprenyl)phenol. Ref.6

Pathway: Cofactor biosynthesis; ubiquinone biosynthesis.

Subcellular location: Mitochondrion matrix

Probable.

Sequence similarities: Belongs to the methyltransferase superfamily. UbiG/COQ3 family.

Sequence caution: The sequence AAF66826.1 differs from that shown. Reason: Frameshift at positions 16, 18, 22, 26, 44, 49, 60, 363 and 364.