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Mouse Connexin 32 Monoclonal Antibody | anti-GJB1 antibody

Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein)

Gene Names
GJB1; CX32
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Purified by affinity chromatography.
Synonyms
Connexin 32; Monoclonal Antibody; Connexin 32 (CX32; Gap Junction Beta 1 Protein; CXB-1; Gap Junction 28kD Liver Protein); Anti -Connexin 32 (CX32; anti-GJB1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10K172
Specificity
Recognizes human Connexin 32. Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
Applicable Applications for anti-GJB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 0.5-1ug/ml detects Connexin 32 in cells and tissues.
Immunohistochemistry (Frozen): 1-2ug/ml detects Connexin 32 in formalin and acetone fixed tissues.
Immunogen
Synthetic peptide corresponding to aa105-123 from human connexin-32. Conjugated to KLH.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-GJB1 antibody
Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of lowmolecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Modulation of gap junction communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, posttranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.
Product Categories/Family for anti-GJB1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
31,787 Da
NCBI Official Full Name
connexin 32
NCBI Official Synonym Full Names
gap junction protein, beta 1, 32kDa<
NCBI Official Symbol
GJB1
NCBI Official Synonym Symbols
CX32
NCBI Protein Information
connexin 32
UniProt Protein Name
Gap junction protein
UniProt Entry Name
Q6URH5_CHICK

Uniprot Description

Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell

By similarity. RuleBase RU000630

Subunit structure: A connexon is composed of a hexamer of connexins

By similarity. RuleBase RU000630

Subcellular location: Membrane; Multi-pass membrane protein

By similarity RuleBase RU000630.

Sequence similarities: Belongs to the connexin family. RuleBase RU000630

Research Articles on GJB1

Similar Products

Product Notes

The GJB1 (Catalog #AAA631932) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Connexin 32 (CX32, Gap Junction Beta 1 Protein, CXB-1, Gap Junction 28kD Liver Protein) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Connexin 32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 0.5-1ug/ml detects Connexin 32 in cells and tissues. Immunohistochemistry (Frozen): 1-2ug/ml detects Connexin 32 in formalin and acetone fixed tissues. Researchers should empirically determine the suitability of the GJB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connexin 32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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