Immunohistochemistry (IHC)
(Human liver melanoma cells stained for C1s (insert is isotype isotype control). |Immunohistochemical staining of paraffin sections with antibody M81)
Mouse anti-Human Complement 1s Monoclonal Antibody
Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified
Synonyms
Complement 1s; Monoclonal Antibody; Complement 1s (Complement Component 1 s subcomponent; Basic proline-rich peptide IB-1; C1 esterase; Complement C1s subcomponent precursor); Anti -Complement 1s (Complement Component 1 s subcomponent; anti-Complement 1s antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
9G173
Specificity
Recognizes an epitope on human C protein activated C1s, a subcomponent of the first component of C (C1). C1s is capable of inhibiting C4 binding and activation.
Purity/Purification
Affinity Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, 0.1% BSA.
Applicable Applications for anti-Complement 1s antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays.
Dilution: Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry: 1:10
Immunoprecipitation: 1:10
Dilution: Flow Cytometry: 1:10
Western Blot: 1:10
Immunohistochemistry: 1:10
Immunoprecipitation: 1:10
Immunogen
Human C1s.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Human liver melanoma cells stained for C1s (insert is isotype isotype control). |Immunohistochemical staining of paraffin sections with antibody M81)
Immunohistochemistry (IHC)
(Human liver melanoma cells stained for C1s (insert is isotype isotype control). |Immunohistochemical staining of paraffin sections with antibody M81)
Related Product Information for anti-Complement 1s antibody
Activated C1s is a glycosylated single-polypeptide zymogen, MW 85 kD. Activation of the proenzyme C1s occurs through cleavage by the active form of C1r. The activated protease, activated C1s, consists of a disulfide-linked H chain and a L chain. Activated C1s is a serine protease and its catalytic site is located in the L chain. Activation of the classical C pathway is triggered by activated C1s which cleaves C4 and C2 to form the C3 convertase, C4bC2a. The epitope recognized by the antibody M81 is domain IV and/or V of the gamma-domain of activated C1s. Monoclonal antibody M81 blocks C4 activation and C4 binding to activated C1s. The antibody reacts around the binding site of C1s and reacts with both active and inactive C1s.
Product Categories/Family for anti-Complement 1s antibody
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Product Notes
The Complement 1s (Catalog #AAA606015) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Complement 1s (Complement Component 1 s subcomponent, Basic proline-rich peptide IB-1, C1 esterase, Complement C1s subcomponent precursor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Complement 1s can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry (frozen and paraffin), Immunoassays. Dilution: Flow Cytometry: 1:10 Western Blot: 1:10 Immunohistochemistry: 1:10 Immunoprecipitation: 1:10. Researchers should empirically determine the suitability of the Complement 1s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement 1s, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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