Mouse CLPP Monoclonal Antibody | anti-CLPP antibody

CLPP antibody

Cat. Num. AAA533612

• Gene Names: CLPP; DFNB81; PRLTS3
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: CLPP antibody was purified by affinity chromatography.
• Synonyms: CLPP; Monoclonal Antibody; CLPP antibody; Monoclonal CLPP; Anti-CLPP; Putative ATP-dependent Clp protease proteolytic subunit mitochondrial; Endopeptidase Clp; ClpP caseinolytic protease; anti-CLPP antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1G9
• Purity/Purification: CLPP antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-CLPP antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:500-2000
• Biological Significance: CLPP belongs to the peptidase family S14. CLPP cleaves peptides in various proteins in a process that requires ATP hydrolysis. CLPP, the catalytic core of the Clp proteolytic complex, is widely involved in many cellular processes via the regulation of intracellular protein quality. CLPP may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates.
• Cross-Reactivity: Human
• Immunogen: CLPP antibody was raised in mouse using a full length recombinant protein of human CLPP (NP_006003) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CLPP protein (Right) or empty vector (Left) detected with CLPP antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CLPP protein in paraffin embedded Human colon tissue using CLPP antibody)

Related Product Information for anti-CLPP antibody

Mouse monoclonal CLPP antibody

Product Categories/Family for anti-CLPP antibody

Nutrition & Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 5174419
• NCBI GeneID: 8192
• NCBI Accession #: NP_006003
• NCBI GenBank Nucleotide #: NM_006012.2
• UniProt Accession #: Q16740; B2R4W5
• Molecular Weight: 30,180 Da
• NCBI Official Full Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• NCBI Official Synonym Full Names: caseinolytic mitochondrial matrix peptidase proteolytic subunit
• NCBI Official Symbol: CLPP
• NCBI Official Synonym Symbols: DFNB81; PRLTS3
• NCBI Protein Information: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• UniProt Protein Name: ATP-dependent Clp protease proteolytic subunit, mitochondrial
• Protein Family: ATP-dependent Clp protease proteolytic
• UniProt Gene Name: CLPP
• UniProt Entry Name: CLPP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq, Jul 2008]

Uniprot Description

CLPP: Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. Clp may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. Belongs to the peptidase S14 family.

Protein type: Protease; Mitochondrial; EC 3.4.21.92

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: peptidase activity; identical protein binding; protein binding; serine-type endopeptidase activity

Biological Process: proteolysis involved in cellular protein catabolic process; protein homooligomerization

Disease: Perrault Syndrome 3