Mouse anti-Chicken ChT1 Monoclonal Antibody
Mouse Anti-Chicken ChT1-BIOT
Reactivity
Chicken
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
ChT1; Monoclonal Antibody; Mouse Anti-Chicken ChT1-BIOT; Mouse Anti-Chicken ChT1 - Biotin; Mouse Anti-Chicken ChT1; anti-ChT1 antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
CT1
Specificity
ChT1 antigen (Mr 63/104 kDa) on chicken thymocytes1
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function.3 CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers.4 CT1 also recognizes quail cortical thymocytes.1
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90% of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive.1-4 Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function.3 CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers.4 CT1 also recognizes quail cortical thymocytes.1
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for anti-ChT1 antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Research Application(s): Identification and enumeration of ChT1+ cells by flow cytometry
In vitro inhibition of T cell differentiation
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
In vitro inhibition of T cell differentiation
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
Chicken thymocytes
Characterization
To ensure optimal performance, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference. Representative flow data are shown on page 2 of this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Chen, C.L., H.T.C. Chanh, and M.D. Cooper. 1984. Chicken thymocyte-specific antigen identified by monoclonal antibodies: ontogeny, tissue distribution and biochemical characterization. Eur. J. Immunol. 14:385.
2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.
3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.
4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.
2. Houssaint, E., E. Dietz, and F.V. Jotereau. 1985. Tissue distribution and ontogenic appearance of a chicken T lymphocyte differentiation marker. Eur. J. Immunol. 15:305-308.
3. Kong, F-K., C.L.H. Chen, and M.D. Cooper. 1998. Thymic function can be accurately monitored by the level of recent T cell emigrants in the circulation. Immunity: 8:97-104.
4. Katevuo, K., B.A., Imhof, R. Boyd., A. Chidgey, A. Bean, D. Dunon, T.W.F. Gobel, and O. Vainio. 1999. ChT1, an Ig superfamily molecule required for T cell differentiation. J. Immunol. 162:5685-5694.
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Product Notes
The ChT1 (Catalog #AAA673981) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken ChT1-BIOT reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's ChT1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Research Application(s): Identification and enumeration of ChT1+ cells by flow cytometry In vitro inhibition of T cell differentiation Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the ChT1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ChT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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