Mouse CENPH Monoclonal Antibody | anti-CENPH antibody

CENPH antibody

Cat. Num. AAA831067

• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: CENPH antibody was purified by affinity chromatography.
• Synonyms: CENPH; Monoclonal Antibody; CENPH antibody; Monoclonal CENPH; Anti-CENPH; Centromere Protein H; NNF1; PMF1; anti-CENPH antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4G6
• Purity/Purification: CENPH antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 247

• Applicable Applications for anti-CENPH antibody: Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: FC: 1:100; WB: 1:500
• Biological Significance: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.
• Cross-Reactivity: Human
• Immunogen: CENPH antibody was raised in mouse using a full length recombinant protein of human CENPH (NP_075060) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry

(Flow Cytometric analysis using CENPH antibodyFlow Cytometric analysis of HEK293T cells transfected with either recombinant CENPH protein (red) or empty vector (blue) stained using CENPH antibody)

Western Blot

(Western Blot analysis using CENPH antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CENPH protein (Right) or empty vector (Left) detected with CENPH antibody)

Related Product Information for anti-CENPH antibody

Mouse monoclonal CENPH antibody

Product Categories/Family for anti-CENPH antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 12597655
• NCBI GeneID: 64946
• NCBI Accession #: NP_075060
• NCBI GenBank Nucleotide #: NM_022909.3
• UniProt Accession #: Q9H3R5; A8K3Y1
• Molecular Weight: 28,481 Da
• NCBI Official Full Name: centromere protein H
• NCBI Official Synonym Full Names: centromere protein H
• NCBI Official Symbol: CENPH
• NCBI Protein Information: centromere protein H
• UniProt Protein Name: Centromere protein H
• Protein Family: Centromere protein
• UniProt Gene Name: CENPH
• UniProt Synonym Gene Names: CENP-H
• UniProt Entry Name: CENPH_HUMAN

NCBI Description

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]

Uniprot Description

CENPH: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Required for chromosome congression and efficiently align the chromosomes on a metaphase plate. Belongs to the centromere protein H family.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: nucleoplasm; kinetochore; nucleus; cytosol

Molecular Function: kinetochore binding; protein binding

Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; mitotic cell cycle; kinetochore organization and biogenesis