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Western Blot (WB) (Western Blot detection against Immunogen (33.37kD).)

Mouse anti-Human BHLHA15 Monoclonal Antibody

BHLHA15 (BHLHB8, MIST1, Class A Basic Helix-loop-helix Protein 15, Class B Basic Helix-loop-helix Protein 8, Muscle, Intestine and Stomach Expression 1)

Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
BHLHA15; Monoclonal Antibody; BHLHA15 (BHLHB8; MIST1; Class A Basic Helix-loop-helix Protein 15; Class B Basic Helix-loop-helix Protein 8; Muscle; Intestine and Stomach Expression 1); Anti -BHLHA15 (BHLHB8; anti-BHLHA15 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1E7
Specificity
Recognizes human BHLHA15.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
QRRLESNERERQRMHKLNNAFQALREVIPHVRADKKLSKIETLTLAKNYIKSLTATILTMSSSRLP
Applicable Applications for anti-BHLHA15 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Immunogen
Partial recombinant protein corresponding to aa75-141 from human BHLHA15 (NP_803238) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (33.37kD).)

Western Blot (WB) (Western Blot detection against Immunogen (33.37kD).)

Testing Data

(Detection limit for recombinant GST tagged BHLHA15 is 1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged BHLHA15 is 1ng/ml as a capture antibody.)
Related Product Information for anti-BHLHA15 antibody
Plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3' (By similarity).
Product Categories/Family for anti-BHLHA15 antibody

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Product Notes

The BHLHA15 (Catalog #AAA649454) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BHLHA15 (BHLHB8, MIST1, Class A Basic Helix-loop-helix Protein 15, Class B Basic Helix-loop-helix Protein 8, Muscle, Intestine and Stomach Expression 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BHLHA15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Researchers should empirically determine the suitability of the BHLHA15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRRLESNERE RQRMHKLNNA FQALREVIPH VRADKKLSKI ETLTLAKNYI KSLTATILTM SSSRLP. It is sometimes possible for the material contained within the vial of "BHLHA15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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