Rabbit CLIC6 Polyclonal Antibody | anti-CLIC6 antibody

CLIC6 polyclonal antibody

Cat. Num. AAA3014795

• Gene Names: CLIC6; CLIC1L
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: CLIC6; Polyclonal Antibody; CLIC6 polyclonal antibody; Chloride intracellular channel protein 6; Parchorin; CLIC1L; anti-CLIC6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: CLIC6 polyclonal antibody detects endogenous levels of CLIC6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLIC6 antibody: Western Blot (WB)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to Human CLIC6
• Preparation and Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of CLIC6 polyclonal antibody at 1:500 dilutionLaneA: A549 whole cell lysateLaneB: L02 whole cell lysateLaneC: AML-12 whole cell lysateLaneD: PC12 whole cell lysate)

Related Product Information for anti-CLIC6 antibody

Chloride intracellular channel 6 (CLIC6) is a probable chloride ion channel belonging to the chloride channel CLIC family. CLIC6 is believed to play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport. 2 essential features distinguish CLIC6 from other members of the family: the CLIC6 protein is significantly longer and the CLIC6 gene contains a GC rich segment, which encodes a 10 amino acid motif repeated 14 times in the amino-terminus. The CLIC6 gene is a rare example of large-scale segmental paralogy in which a large (approximately 500 kb) segment on human chromosome (HC) 21 (21q22) is triplicated on HC 1 and HC 6. CLIC6 is also known to interact with dopamine receptors DRD2, DRD3 and DRD4. CLIC6 is primarily expressed in the cytoplasm, however, upon chloride ion efflux from the cell, CLIC6 is translocated to the plasma membrane. CLIC6 has been identified in brain, placenta, pancreas and liver. CLIC6 is a 704 amino acid protein and there are 2 known isoforms, A and B. CLIC6 is also identified as parchorin in rabbit.

NCBI and Uniprot Product Information

• NCBI GI #: 27894378
• NCBI GeneID: 54102
• NCBI Accession #: NP_444507.1
• NCBI GenBank Nucleotide #: NM_053277.1
• UniProt Accession #: Q96NY7; Q8IX31; A8K0U8
• Molecular Weight: ~73 kDa
• NCBI Official Full Name: chloride intracellular channel protein 6
• NCBI Official Synonym Full Names: chloride intracellular channel 6
• NCBI Official Symbol: CLIC6
• NCBI Official Synonym Symbols: CLIC1L
• NCBI Protein Information: chloride intracellular channel protein 6; parchorin; chloride channel form A
• UniProt Protein Name: Chloride intracellular channel protein 6
• Protein Family: Chloride intracellular channel protein
• UniProt Gene Name: CLIC6
• UniProt Synonym Gene Names: CLIC1L
• UniProt Entry Name: CLIC6_HUMAN

NCBI Description

This gene encodes a member of the chloride intracellular channel family of proteins. The gene is part of a large triplicated region found on chromosomes 1, 6, and 21. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

CLIC6: May insert into membranes and form chloride ion channels. May play a critical role in water-secreting cells, possibly through the regulation of chloride ion transport. Belongs to the chloride channel CLIC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Channel, chloride; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.12

Cellular Component: cytoplasm; plasma membrane

Molecular Function: protein C-terminus binding; chloride channel activity; protein homodimerization activity; voltage-gated ion channel activity; D4 dopamine receptor binding; D3 dopamine receptor binding; D2 dopamine receptor binding