Mouse fibroblast growth factor 5 ELISA Kit | FGF5 elisa kit

Mouse Fibroblast growth factor 5, FGF5 ELISA Kit

Cat. Num. AAA931255

• Gene Names: Fgf5; go; Fgf-5; HBGF-5; angora
• Reactivity: Mouse
• Synonyms: fibroblast growth factor 5; Mouse Fibroblast growth factor 5; FGF5 ELISA Kit; Mouse Fibroblast growth factor 5 (FGF5) ELISA kit; HBGF-5; Smag-82; heparin-binding growth factor 5; FGF5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FGF5. No significant cross-reactivity or interference between mouse FGF5 and analogues was observed.
• Sequence Length: 121

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: < 7.8 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF5 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 471270260
• NCBI GeneID: 14176
• NCBI Accession #: NP_001264197.1
• NCBI GenBank Nucleotide #: NM_001277268.1
• UniProt Accession #: P15656; O88825
• Molecular Weight: 29,103 Da
• NCBI Official Full Name: fibroblast growth factor 5 isoform 2
• NCBI Official Synonym Full Names: fibroblast growth factor 5
• NCBI Official Symbol: Fgf5
• NCBI Official Synonym Symbols: go; Fgf-5; HBGF-5; angora
• NCBI Protein Information: fibroblast growth factor 5; heparin-binding growth factor 5
• UniProt Protein Name: Fibroblast growth factor 5
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf5
• UniProt Synonym Gene Names: Fgf-5; FGF-5; HBGF-5
• UniProt Entry Name: FGF5_MOUSE

NCBI Description

This gene encodes a secreted protein that is a member of a family of heparin-binding growth factors. The encoded protein regulates cell proliferation, particularly the growth of hair follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

FGF5: Plays an important role in the regulation of cell proliferation and cell differentiation. Required for normal regulation of the hair growth cycle. Functions as an inhibitor of hair elongation by promoting progression from anagen, the growth phase of the hair follicle, into catagen the apoptosis-induced regression phase. Belongs to the heparin-binding growth factors family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Oncoprotein; Cytokine

Cellular Component: extracellular region

Molecular Function: growth factor activity; receptor binding; fibroblast growth factor receptor binding

Biological Process: cell proliferation; fibroblast growth factor receptor signaling pathway; glial cell differentiation; positive regulation of cell division; positive regulation of cell proliferation