Human Heat shock protein HSP 90-alpha ELISA Kit | HSP90AA1 elisa kit

Human Heat shock protein HSP 90-alpha ELISA Kit

Cat. Num. AAA762438

• Gene Names: HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; HEL-S-65p
• Reactivity: Human
• Synonyms: Heat shock protein HSP 90-alpha; Human Heat shock protein HSP 90-alpha ELISA Kit; HSP-90 (Heat Shock Protein 90)/HSP90AA1/Hsp90/HSP86/HSP89A/HSP90A/HSP90N/HSPC1/HSPCA/HSPCAL1/HSPCAL4/HSPN/LAP2/FLJ31884/Heat shock 86 kDa/heat shock 90kD protein 1; alpha/heat shock 90kD protein 1; alpha-like 4/heat shock 90kD protein; alpha-like 4/heat shock protein HSP 90-alpha/Hsp89; HSP90AA1 elisa kit

• Reactivity: Human
• Sequence Length: 732

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 3.125-200ng/ml
• Sensitivity: 1.875ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HSP90AA1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HSP-90 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HSP-90 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HSP-90 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HSP-90 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 154146191
• NCBI GeneID: 3320
• NCBI Accession #: NP_005339.3
• NCBI GenBank Nucleotide #: NM_005348.3
• Molecular Weight: 98,161 Da
• NCBI Official Full Name: heat shock protein HSP 90-alpha isoform 2
• NCBI Official Synonym Full Names: heat shock protein 90kDa alpha family class A member 1
• NCBI Official Symbol: HSP90AA1
• NCBI Official Synonym Symbols: EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4; HEL-S-65p
• NCBI Protein Information: heat shock protein HSP 90-alpha
• UniProt Protein Name: Heat shock protein HSP 90-alpha
• Protein Family: Heat shock protein
• UniProt Gene Name: HSP90AA1
• UniProt Synonym Gene Names: HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2
• UniProt Entry Name: HS90A_HUMAN

NCBI Description

The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

HSP90A: a molecular chaperone of the heat shock protein 90 family. Has ATPase activity. Known to interact with a wide variety of proteins including steroid hormone receptors, neuropeptide Y, FKBP51/54, and FKBP52. G protein-coupled receptor kinases are stabilized by interacting with HSP 90. Hsp70 and Hsp90 promote tau solubility and tau binding to microtubules, reducing insoluble tau phosphorylation of tau.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: cytoplasm; cytosol; extracellular region; lysosomal lumen; membrane; nucleoplasm; nucleus; plasma membrane

Molecular Function: ATP binding; ATPase activity; GTPase binding; histone deacetylase binding; identical protein binding; nitric-oxide synthase regulator activity; nucleotide binding; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; TPR domain binding

Biological Process: chaperone-mediated protein complex assembly; G2/M transition of mitotic cell cycle; mitochondrial transport; positive regulation of nitric oxide biosynthetic process; protein import into mitochondrial outer membrane; protein refolding; protein stabilization; receptor-mediated endocytosis; regulation of nitric-oxide synthase activity; regulation of protein ubiquitination; response to antibiotic; response to cold; response to heat; vascular endothelial growth factor receptor signaling pathway