Rabbit anti-Mouse CDH5  Monoclonal Antibody | anti-CDH5  antibody

Rabbit anti Mouse CDH5  Monoclonal Antibody

Cat. Num. AAA2544510

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: CDH5 ; Monoclonal Antibody; Rabbit anti Mouse CDH5 Monoclonal Antibody; anti-CDH5 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 669

• Applicable Applications for anti-CDH5 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Mouse CDH5. The detection limit for Mouse CDH5 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Mouse VE-Cadherin/CD144/CDH5 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CDH5  antibody

Cadherins (Calcium dependent adhesion molecules) are a class of transmembrane proteins. Cadherin-5, also known as VE-cadherin, CDH5 and CD144, an endothelial specific cell-cell adhesion molecule, plays a pivotal role in the formation, maturation and remodeling of the vascular wall. VE-Cadherin is widely considered to be specific for vascular endothelia in which it is either the sole or the predominant cadherin, often co-existing with N-cadherin. This specificity of VE-cadherin for vascular endothelial cells is important not only in blood and lymph vessel biology and medicine, but also for cell-type-based identifys, notably those of metastatic tumors. As a classical cadherin, VE-Cadherin links endothelial cells together by homophilic interactions mediated by its extracellular part and associates intracellularly with the actin cytoskeleton via catenins. Mechanisms that regulate VE-cadherin-mediated adhesion are important for the control of vascular permeability and leukocyte extravasation. In addition to its adhesive functions, VE-Cadherin regulates various cellular processes such as cell proliferation and apoptosis and modulates vascular endothelial growth factor receptor functions. Consequently, VE-cadherin is essential during embryonic angiogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 109730054
• NCBI GeneID: 1003
• Molecular Weight: 74,499 Da
• NCBI Official Full Name: CDH5 protein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cell junction; cell surface; intercellular junction; membrane; plasma membrane

Molecular Function: beta-catenin binding; protein binding; receptor binding

Biological Process: intercellular junction assembly; transforming growth factor beta receptor signaling pathway