Rabbit anti-Human Sphingomyelin Synthase 2 Polyclonal Antibody | anti-SGMS2 antibody

Sphingomyelin Synthase 2

Cat. Num. AAA395922

• Gene Names: SGMS2; SMS2
• Reactivity: Human
• Applications: Western Blot
• Purity: Antigen Immunoaffiinity Purification
• Synonyms: Sphingomyelin Synthase 2; Polyclonal Antibody; Phosphatidylcholine:ceramide cholinephosphotransferase 2; Sphingomyelin synthase 2; SMS2; anti-SGMS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Immunoaffiinity Purification
• Form/Format: Purified IgG in phosphate buffered saline, pH 7.4
• Sequence Length: 365

• Applicable Applications for anti-SGMS2 antibody: Western Blot (WB)
• Immunogen: Synthetic peptide derived from the human sphingomyelin synthase 2 protein.
• Positive Control: Brain, heart, kidney, liver, muscle and stomach.
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Immunohistochemistry (IHC)

(Fig 1. Western blot on human brain lysate (10 ug/lane) using X2059P antibody to Sphingomyelin Synthase 2 (0.5 ug/ml). Lane A] 1 ug/ml antibody alone, lane B] antibody plus 3 ug blocking peptide. Blot was developed with Pierce?s Super Sytem West Femto - 1 minute exposure. Fig 2. Human kidney section stained with anti-SMS-2 antibody (Cat X2059P) at 2 ug/ml. Anti-rabbit Ig H+L HRP conjugate (5 ug/ml) without antigen retrieval.)

Related Product Information for anti-SGMS2 antibody

Sphingomyelin (SM) is a major component of animal plasma membranes. Its production involves the transfer of phosphocholine from phosphatidylcholine and then onto ceramide. This also yields diacylglycerol as a side product. The reaction is catalysed by SM synthase, an important enzyme in the regulation of diacylglycerol and ceramide as anti- and proapoptotic stimuli. SM synthesis occurs in the lumen of the Golgi as well as on or at the cell surface. It is uncertain which SM enzyme forms are present at these differnet cellular locations. Human, mouse and Caenorhabditis elegans genomes each contain at least two different SM synthase (SMS) genes. Human SMS1 is localised to the Golgi, SMS2 resides primarily at the plasma membrane.

Product Categories/Family for anti-SGMS2 antibody

Antigen Immunoaffinity Purified Polyclonal; Cell Communication

NCBI and Uniprot Product Information

• NCBI GI #: 210147407
• NCBI GeneID: 166929
• NCBI Accession #: NP_001129729.1
• NCBI GenBank Nucleotide #: NM_001136257.1
• UniProt Accession #: Q8NHU3; A8K2S9; B2RA61
• Molecular Weight: 42 kDa
• NCBI Official Full Name: phosphatidylcholine:ceramide cholinephosphotransferase 2
• NCBI Official Synonym Full Names: sphingomyelin synthase 2
• NCBI Official Symbol: SGMS2
• NCBI Official Synonym Symbols: SMS2
• NCBI Protein Information: phosphatidylcholine:ceramide cholinephosphotransferase 2; SM synthase
• UniProt Protein Name: Phosphatidylcholine:ceramide cholinephosphotransferase 2
• UniProt Gene Name: SGMS2
• UniProt Synonym Gene Names: SMS2
• UniProt Entry Name: SMS2_HUMAN

NCBI Description

Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined.[provided by RefSeq, Oct 2008]

Uniprot Description

Function: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Plasma membrane SMS2 can also convert phosphatidylethanolamine (PE) to ceramide phosphatidylethanolamine (CPE). Major form in liver. Required for cell growth in certain cell types. Regulator of cell surface levels of ceramide, an important mediator of signal transduction and apoptosis. Regulation of sphingomyelin (SM) levels at the cell surface affects insulin sensitivity. Ref.6 Ref.8

Catalytic activity: A ceramide + a phosphatidylcholine = a sphingomyelin + a 1,2-diacyl-sn-glycerol.

Enzyme regulation: Inhibited by bacterial PC-phospholipase C inhibitor D609. Ref.6

Subcellular location: Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note: Predominantly plasma membrane, some in Golgi apparatus. Some localization in the perinuclear region where it colocalizes with a sialyltransferase. Ref.6 Ref.9 Ref.10

Tissue specificity: Brain, heart, kidney, liver, muscle and stomach. Also expressed in a number of cell lines such as carcinoma HeLa cells, hepatoma Hep-G2 cells, and colon carcinoma Caco-2 cells. Ref.6

Post-translational modification: Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization. Ref.10

Miscellaneous: Overexpression of the human protein in mouse causes increased non-HDL-sphingomyelin and non-HDL cholesterol levels, decreased HDL-sphingomyelin and HDL-cholesterol levels and increases lipoprotein atherogenic potential.

Sequence similarities: Belongs to the sphingomyelin synthase family.