Rabbit anti-Human CDH1  Monoclonal Antibody | anti-CDH1  antibody

Rabbit anti Human CDH1  Monoclonal Antibody

Cat. Num. AAA2543880

• Gene Names: CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• Reactivity: Human
• Applications: ELISA
• Synonyms: CDH1 ; Monoclonal Antibody; Rabbit anti Human CDH1 Monoclonal Antibody; anti-CDH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 647

• Applicable Applications for anti-CDH1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human E-cad. The detection limit for Human E-cad is approximately 0.156 ng/well.
• Immunogen: Recombinant Human E-cadherin/CDH1/Cadherin-1/E-Cad protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CDH1  antibody

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

NCBI and Uniprot Product Information

• NCBI GI #: 148921649
• NCBI GeneID: 999
• Molecular Weight: 90,942 Da
• NCBI Official Full Name: CDH1 protein
• NCBI Official Synonym Full Names: cadherin 1
• NCBI Official Symbol: CDH1
• NCBI Official Synonym Symbols: UVO; CDHE; ECAD; LCAM; Arc-1; CD324
• NCBI Protein Information: cadherin-1
• UniProt Protein Name: Cadherin-1
• UniProt Gene Name: CDH1
• UniProt Synonym Gene Names: CDHE; UVO; E-cadherin
• UniProt Entry Name: CADH1_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH1: a single-pass type I membrane protein, and calcium dependent cell adhesion proteins. It is a ligand for integrin alpha-E/beta-7, and it colocalizes with DLG7 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis. Has a potent invasive suppressor role.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: actin cytoskeleton; apical junction complex; catenin complex; cell junction; cell-cell adherens junction; cortical actin cytoskeleton; cytoplasm; extracellular region; flotillin complex; focal adhesion; integral to membrane; internal side of plasma membrane; lamellipodium; lateral plasma membrane; perinuclear region of cytoplasm; plasma membrane; trans-Golgi network

Molecular Function: ankyrin binding; beta-catenin binding; cell adhesion molecule binding; gamma-catenin binding; glycoprotein binding; GTPase activating protein binding; protein binding

Biological Process: cell-cell adhesion; extracellular matrix disassembly; extracellular matrix organization and biogenesis; negative regulation of cell-cell adhesion; positive regulation of transcription factor import into nucleus; positive regulation of transcription, DNA-dependent

Disease: Breast Cancer; Endometrial Cancer; Gastric Cancer, Hereditary Diffuse; Ovarian Cancer; Prostate Cancer