Mouse cdc45-Related Protein Monoclonal Antibody | anti-CDC45 antibody

cdc45-Related Protein (cdc45L, cdc45L2, cdc45-like, PORC-PI-1)

Cat. Num. AAA600309

• Gene Names: CDC45; CDC45L; CDC45L2; PORC-PI-1
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: cdc45-Related Protein; Monoclonal Antibody; cdc45-Related Protein (cdc45L; cdc45L2; cdc45-like; PORC-PI-1); Anti -cdc45-Related Protein (cdc45L; anti-CDC45 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C66
• Specificity: Recognizes human cdc45-Related Protein.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-CDC45 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human cdc45-Related Protein
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-CDC45 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 4502713
• NCBI GeneID: 8318
• NCBI Accession #: NP_003495.1
• NCBI GenBank Nucleotide #: NM_003504.3
• UniProt Accession #: O75419; O60856; Q20WK8; Q6UW54; Q9UP68
• Molecular Weight: 65,569 Da
• NCBI Official Full Name: cell division control protein 45 homolog isoform 2
• NCBI Official Synonym Full Names: cell division cycle 45 homolog (S. cerevisiae)
• NCBI Official Symbol: CDC45
• NCBI Official Synonym Symbols: CDC45L; CDC45L2; PORC-PI-1
• NCBI Protein Information: cell division control protein 45 homolog; human CDC45; OTTHUMP00000197247; OTTHUMP00000197248; CDC45-related protein; cell division cycle 45-like 2; CDC45 cell division cycle 45 homolog
• UniProt Protein Name: Cell division control protein 45 homolog
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC45
• UniProt Synonym Gene Names: CDC45L; CDC45L2
• UniProt Entry Name: CDC45_HUMAN

NCBI Description

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus

Molecular Function: protein binding; 3'-5' DNA helicase activity; DNA replication origin binding; chromatin binding; single-stranded DNA binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; DNA replication checkpoint; DNA duplex unwinding; G1/S transition of mitotic cell cycle; pre-replicative complex assembly; double-strand break repair via break-induced replication