Rabbit Cdc45 Polyclonal Antibody | anti-CDC45 antibody

Cdc45

Cat. Num. AAA619873

• Gene Names: CDC45; CDC45L; CDC45L2; PORC-PI-1
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Cdc45; Polyclonal Antibody; Anti -Cdc45; anti-CDC45 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Cdc45. Species crossreactivity: mouse, rat, monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES (pH 7.5), 150mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-CDC45 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic peptide corresponding to the carboxy terminus of human cdc45 protein (KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell types using MBS619873.)

Related Product Information for anti-CDC45 antibody

The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle. Origins of replication are dispersed throughout the genome and their activities are regulated via the sequential binding of pre-replication and replication factors. The origin recognition complex (ORC) is thought to bind to chromatin throughout the cell cycle (1,2). The pre-replication complex (Pre-RC) forms in late mitosis/early G1 phase with the binding of CDT1 and cdc6 to the origin, which allows binding of the heterohexameric MCM2-7 complex. The MCM complex is thought to be the replicative helicase and formation of the pre-RC is referred to as chromatin licensing. Subsequent initiation of DNA replication requires the activation of the S-phase promoting kinases cdk2 and cdc7. Cdc7 phosphorylates MCM proteins bound to chromatin and, in conjunction with CDT1, recruits the replication factor cdc45 (3-5). Cdc45 is required for formation of pre-initiation com- plexes at the G1/S transition and for activation of replication origins. The level of cdc45 protein expression is associated with the proliferative status of the cell or tissue. Terminally differentiated and senescent cells lack cdc45 and highly proliferative cell lines express high levels of cdc45 (6).

Product Categories/Family for anti-CDC45 antibody

Antibodies; Abs to Replication Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4502713
• NCBI GeneID: 8318
• NCBI Accession #: NP_003495.1
• NCBI GenBank Nucleotide #: NM_003504.3
• UniProt Accession #: O75419; O60856; Q20WK8; Q6UW54; Q9UP68
• Molecular Weight: 65,569 Da
• NCBI Official Full Name: cell division control protein 45 homolog isoform 2
• NCBI Official Synonym Full Names: cell division cycle 45 homolog (S. cerevisiae)
• NCBI Official Symbol: CDC45
• NCBI Official Synonym Symbols: CDC45L; CDC45L2; PORC-PI-1
• NCBI Protein Information: cell division control protein 45 homolog; human CDC45; OTTHUMP00000197247; OTTHUMP00000197248; CDC45-related protein; cell division cycle 45-like 2; CDC45 cell division cycle 45 homolog
• UniProt Protein Name: Cell division control protein 45 homolog
• Protein Family: Cell division control protein
• UniProt Gene Name: CDC45
• UniProt Synonym Gene Names: CDC45L; CDC45L2
• UniProt Entry Name: CDC45_HUMAN

NCBI Description

The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

CDC45L: Required for initiation of chromosomal DNA replication. Associated with ORC2. Widely expressed, highest levels are found in adult testis and thymus and in fetal liver. Belongs to the CDC45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus

Molecular Function: protein binding; 3'-5' DNA helicase activity; chromatin binding; DNA replication origin binding; single-stranded DNA binding

Biological Process: G1/S-specific transcription in mitotic cell cycle; DNA replication initiation; regulation of chromatin silencing at telomere; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication checkpoint; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle; pre-replicative complex assembly; double-strand break repair via break-induced replication