Mouse anti-Human CDC2 Monoclonal Antibody | anti-CDC2 antibody

CDC2 (Cell Division Control Protein 2 Homolog, CDC28A, Cell Division Protein Kinase 1, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, P34CDC2) (PE)

Cat. Num. AAA6157004

• Gene Names: CDK1; CDC2; CDC28A; P34CDC2
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CDC2; Monoclonal Antibody; CDC2 (Cell Division Control Protein 2 Homolog; CDC28A; Cell Division Protein Kinase 1; Cyclin-dependent Kinase 1; CDK1; p34 Protein Kinase; P34CDC2) (PE); anti-CDC2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1A4-1A9
• Specificity: Recognizes human CDC2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1119

• Applicable Applications for anti-CDC2 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-297 from human CDC2 (AAH14563) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MEDYTKIEKIGEGTYGVVYKGRHKTTGQVVAMKKIRLESEEEGVPSTAIREISLLKELRHPNIVSLQDVLMQDSRLYLIFEFLSMDLKKYLDSIPPGQYMDSSLVKSYLYQILQGIVFCHSRRVLHRDLKPQNLLIDDKGTIKLADFGLARAFGIPIRVYTHEVVTLWYRSPEVLLGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (58.41kD).)

Western Blot (WB)

(Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)

Western Blot (WB)

(CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)

Product Categories/Family for anti-CDC2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 15778966
• NCBI GeneID: 983
• NCBI Official Full Name: Homo sapiens cell division cycle 2, G1 to S and G2 to M, mRNA
• NCBI Official Synonym Full Names: cyclin dependent kinase 1
• NCBI Official Symbol: CDK1
• NCBI Official Synonym Symbols: CDC2; CDC28A; P34CDC2
• NCBI Protein Information: cyclin-dependent kinase 1
• Protein Family: CDC25-like phosphatase

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]