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Western Blot (WB) (Western Blot: Sample: Mouse Liver Tissue.)

Mouse anti-Mouse Apolipoprotein A5 (APOA5) Monoclonal Antibody | anti-APOA5 antibody

Monoclonal Antibody to Apolipoprotein A5 (APOA5)

Gene Names
Apoa5; RAP3; Apoav; 1300007O05Rik
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Apolipoprotein A5 (APOA5); Monoclonal Antibody; Monoclonal Antibody to Apolipoprotein A5 (APOA5); Apo-A5; APOA-V; APOAV; RAP3; Regeneration-associated protein 3; anti-APOA5 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C6
Specificity
The antibody is a mouse monoclonal antibody raised against APOA5. It has been selected for its ability to recognize APOA5 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-APOA5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Immunogen
Recombinant Apolipoprotein A5 (APOA5) (Arg21-Pro210)
Cross Reactivity
Mouse
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Label
None
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2061831
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Liver Tissue.)

Western Blot (WB) (Western Blot: Sample: Mouse Liver Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant APOA5, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant APOA5, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Liver Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,262 Da
NCBI Official Full Name
apolipoprotein A-V
NCBI Official Synonym Full Names
apolipoprotein A-V
NCBI Official Symbol
Apoa5
NCBI Official Synonym Symbols
RAP3; Apoav; 1300007O05Rik
NCBI Protein Information
apolipoprotein A-V
UniProt Protein Name
Apolipoprotein A-V
Protein Family
UniProt Gene Name
Apoa5
UniProt Synonym Gene Names
Rap3; Apo-AV; ApoA-V

Uniprot Description

APOA5: Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and a inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Interacts with GPIHBP1. Up-regulated by PPARA agonists, which are used clinically to lower serum TG (such as fibrates). Liver and plasma. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9|9 A5.2

Cellular Component: chylomicron; extracellular region; extracellular space

Molecular Function: cholesterol binding; cholesterol transporter activity; enzyme activator activity; enzyme binding; heparin binding; lipid binding; low-density lipoprotein receptor binding; phosphatidylcholine binding; phospholipid binding

Biological Process: acylglycerol homeostasis; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; lipid transport; lipoprotein metabolic process; neuron projection regeneration; phosphatidylcholine metabolic process; phospholipid efflux; positive regulation of fatty acid biosynthetic process; positive regulation of lipid catabolic process; positive regulation of lipoprotein lipase activity; regulation of intestinal cholesterol absorption; reverse cholesterol transport; transport; triacylglycerol metabolic process; triglyceride catabolic process

Research Articles on APOA5

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Product Notes

The APOA5 apoa5 (Catalog #AAA2085929) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Apolipoprotein A5 (APOA5) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Apolipoprotein A5 (APOA5) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the APOA5 apoa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein A5 (APOA5), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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