Mouse CD99 Monoclonal Antibody | anti-CD99 antibody

MOUSE ANTI HUMAN CD99:FITC

Cat. Num. AAA215283

• Gene Names: CD99; MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD99; Monoclonal Antibody; MOUSE ANTI HUMAN CD99:FITC; anti-CD99 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: DN16
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 169

• Applicable Applications for anti-CD99 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD99 antibody

Mouse anti human CD99 antibody, clone DN16 recognizes human CD99, also known as E2 antigen, MIC2 or 12E7. CD99 is a 185 amino acid ~32kDa single pass type I transmembrane O-glycosylated glycoprotein. Three isoforms can be producted by alternative splicing. Epitope analysis of the DN16 clone suggests the antibody recognizes a minimal peptide sequence "LPDNENKK" located between residues 32 and 39 towards the N-terminal region of the molecule. This sequence is present in both isoforms I and II but is largely absent from isoform 3 suggesting that the antibody will only recognize isoforms I and II (Gil et al. 2002). CD99 expression is notable in the testis, pancreas, bone marrow, lymph nodes and spleen. CD99 is expressed on all classes of leukocytes and tends to be highest on immature cells. Functionally CD99 has been found to be involved in cellular adhesion/aggregation (Krisanaprakornkit et al. 2013) and apoptosis (Sciandra et al. 2014).

NCBI and Uniprot Product Information

• NCBI GI #: 171543879
• NCBI GeneID: 4267
• NCBI Accession #: NP_001116370.1
• NCBI GenBank Nucleotide #: NM_001122898.1
• UniProt Accession #: P14209; O00518; Q6ICV7; A6NIW1
• Molecular Weight: 17,128 Da
• NCBI Official Full Name: CD99 antigen isoform b
• NCBI Official Synonym Full Names: CD99 molecule
• NCBI Official Symbol: CD99
• NCBI Official Synonym Symbols: MIC2; HBA71; MIC2X; MIC2Y; MSK5X
• NCBI Protein Information: CD99 antigen; E2 antigen; MIC2 (monoclonal antibody 12E7); T-cell surface glycoprotein E2; antigen identified by monoclonal 12E7, Y homolog; antigen identified by monoclonal antibodies 12E7, F21 and O13; surface antigen MIC2
• UniProt Protein Name: CD99 antigen
• Protein Family: CD99 antigen
• UniProt Gene Name: CD99
• UniProt Synonym Gene Names: MIC2; MIC2X; MIC2Y
• UniProt Entry Name: CD99_HUMAN

NCBI Description

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2013]

Uniprot Description

CD99: Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes. Belongs to the CD99 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.32 and Yp11.3

Cellular Component: focal adhesion; integral to plasma membrane; cytoplasm; plasma membrane

Biological Process: cell adhesion