Mouse anti-Human CD98 Monoclonal Antibody | anti-CD98 antibody

anti-human CD98-Biotin

Cat. Num. AAA666078

• Gene Names: SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD98; Monoclonal Antibody; anti-human CD98-Biotin; Monoclonal anti-human CD98(4F2)/Biotin; anti-CD98 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: UM7F8
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 507

• Applicable Applications for anti-CD98 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured Jurkat cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD98/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.89 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD98 antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Molt 13 T cell line
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD98 antibody

Information: Human CD98 (4F2) is expressed on monocytes and activated cells. All cultured human cell lines tested express CD98. CD98 is thought to be an activation antigen. Antibody UM7F8 recognizes a 125 kd heterodimeric membrane glycoprotein CD98. Antibody UM7F8 is comitogenic with soluble anti-CD2 and immoblized anti-CD3 mAbs.

References

A.W. Friedman, et al, (1994) Cell Immunol 154: 253-263. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 280-283.

NCBI and Uniprot Product Information

• NCBI GI #: 3767584
• NCBI GeneID: 8140
• Molecular Weight: 55,010 Da
• NCBI Official Full Name: CD98 light chain
• NCBI Official Synonym Full Names: solute carrier family 7 (amino acid transporter light chain, L system), member 5
• NCBI Official Symbol: SLC7A5
• NCBI Official Synonym Symbols: E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
• NCBI Protein Information: large neutral amino acids transporter small subunit 1; 4F2 LC; 4F2 light chain; CD98 light chain; integral membrane protein E16; L-type amino acid transporter 1; solute carrier family 7 member 5; large neutral amino acids transporter 1; y+ system cationic amino acid transporter; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• UniProt Protein Name: Large neutral amino acids transporter small subunit 1
• UniProt Gene Name: SLC7A5
• UniProt Synonym Gene Names: CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1
• UniProt Entry Name: LAT1_HUMAN

Uniprot Description

SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; apical plasma membrane; integral to membrane; nucleolus; plasma membrane; cytosol; nucleus

Molecular Function: L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; transport; amino acid transport; ion transport; blood coagulation; cell differentiation; transmembrane transport; leukocyte migration