Rabbit anti-Mouse, Rat BMI-1 Polyclonal Antibody | anti-BMI-1 antibody

RABBIT ANTI BMI-1

Cat. Num. AAA221477

• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: BMI-1; Polyclonal Antibody; RABBIT ANTI BMI-1; anti-BMI-1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 469

• Applicable Applications for anti-BMI-1 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: 15 amino acid peptide sequence near the centre of human BMI-1.; Buffer Solution
• Antiserum Preparation: Antisera to human BMI-1 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from K562 human erythromyeloblastoid cells probed with Rabbit anti Human BMI-1 (MBS221477) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunocytochemical staining of K562 cells with Rabbit anti Human BMI-1 (MBS221477))

Related Product Information for anti-BMI-1 antibody

Rabbit anti BMI-1 antibody recognizes 37kDa polycomb complex protein BMI-1, involved in segment specification, cell growth and maintenance, transcriptional regulation and chromatin modification. BMI-1 is a proto-oncogene which cooperates with the myc oncogene to produce B-lymphomas. It is required in the regulation of normal adult and leukaemic stem cells, making BMI-1 a potential target for stem cell proliferation and anti-cancer therapies. BMI-1 represses transcription at the ink4 locus which contains tumour suppressors p16 and p19Arf. BMI-1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex which ubiquitinates histone-2A at lys119, resulting in a heritable change in the ability of chromatin to express genes. BMI-1 is also required to stimulate the E3 ubiquitin ligase activity of RNF2/RING2 within the PRC1 complex. The Notch ligand DLL4 (delta-like protein 4) enhances T cell proliferation, by directly influencing BMI-1 transcription.

NCBI and Uniprot Product Information

• NCBI GI #: 323276616
• NCBI GeneID: 100532731
• NCBI Accession #: NP_001190991.1
• NCBI GenBank Nucleotide #: NM_001204062.1
• UniProt Accession #: P35226; Q16030; Q5T8Z3; Q96F37
• Molecular Weight: 36,949 Da
• NCBI Official Full Name: COMMD3-BMI1 read-through protein
• NCBI Official Synonym Full Names: COMMD3-BMI1 readthrough
• NCBI Official Symbol: COMMD3-BMI1
• NCBI Protein Information: COMMD3-BMI1 read-through protein
• UniProt Protein Name: Polycomb complex protein BMI-1
• UniProt Gene Name: BMI1
• UniProt Synonym Gene Names: PCGF4; RNF51
• UniProt Entry Name: BMI1_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

Uniprot Description

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.

Protein type: Oncoprotein; EC 6.3.2.-; Motility/polarity/chemotaxis; Ubiquitin conjugating system; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: nucleoplasm; heterochromatin; nuclear body; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding

Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; DNA methylation; positive regulation of B cell proliferation; somatic stem cell division; hemopoiesis; brain development; histone acetylation