Mouse anti-Human CD97 Monoclonal Antibody | anti-CD97 antibody

CD97 (CD97 Antigen, CD97 Molecule, Leukocyte Antigen CD97, Seven Transmembrane Heterodimeric Receptor Associated with Inflammation, TM7LN1) (HRP)

Cat. Num. AAA6151697

• Gene Names: ADGRE5; CD97; TM7LN1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CD97; Monoclonal Antibody; CD97 (CD97 Antigen; CD97 Molecule; Leukocyte Antigen CD97; Seven Transmembrane Heterodimeric Receptor Associated with Inflammation; TM7LN1) (HRP); anti-CD97 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 5D5
• Specificity: Recognizes human CD97.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 835

• Applicable Applications for anti-CD97 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa421-529 from human CD97 (NP_510966) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: KKQAELEEIYESSIRGVQLRRLSAVNSIFLSHNNTKELNSPILFAFSHLESSDGEAGRDPPAKDVMPGPRQELLCAFWKSDSDRGGHWATEGCQVLGSKNGSTTCQCSH
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (37.73kD).)

Western Blot (WB)

(Western Blot analysis of CD97 expression in transfected 293T cell line by CD97 monoclonal antibody. Lane 1: CD97 transfected lysate (81.7kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged CD97 is ~0.1ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of CD97 over-expressed 293 cell line, cotransfected with CD97 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CD97 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Related Product Information for anti-CD97 antibody

CD97, a CD55 Receptor, is a leukocyte antigen located on most activated leukocytes. CD97 is highly homologous to EMR2 with five EGF-like domains in the N-terminal extracellular domain, suggesting that it may be involved in cell signaling as well as in cell adhesion (Hamann et al., 1996). Two variants are produced by alternative splicing, where the shorter form lacks exons 5 and 6 and reduces the number of EGF-like domains from 5 to 3, compared to the full-length isoform. CD97 is associated with with the activated lesions of multiple sclerosis. CD97 expression is also implicated in the dedifferentiation of colon and thyroid tumors. CD97 is expressed abundantly in cells of hematopoietic origin and is markedly upregulated on activated T and B cells. ESTs have been isolated from a wide variety of tissue libraries.

Product Categories/Family for anti-CD97 antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 17978491
• NCBI GeneID: 976
• NCBI Accession #: NP_510966
• NCBI GenBank Nucleotide #: NM_078481
• UniProt Accession #: P48960
• NCBI Official Full Name: CD97 antigen isoform 1 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• Protein Family: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: extracellular space; focal adhesion; membrane; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; protein binding; transmembrane receptor activity; calcium ion binding

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; cell-cell signaling; immune response; cell motility; inflammatory response; cell adhesion