Human CD97 PicoKine ELISA Kit | ADGRE5 elisa kit

Human CD97 PicoKine ELISA Kit

Cat. Num. AAA177600

• Gene Names: ADGRE5; CD97; TM7LN1
• Reactivity: Human
• Synonyms: CD97 PicoKine; Human CD97 PicoKine ELISA Kit; CD97 antigen; CD 97; CD_antigen=CD97; CD97; CD97 antigen subunit beta; CD97 molecule; CD97_HUMAN; Leukocyte antigen CD97; Seven span transmembrane protein; Seven transmembrane helix receptor; Seven transmembrane heterodimeric receptor associated with inflammation; TM7LN 1; TM7LN1 ; adhesion G protein-coupled receptor E5; ADGRE5 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CD97
• Sequence Length: 786

• Assay Range: 125pg/ml-8000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of human CD97 in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: NSO, Q21-Q398
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml human CD97 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD97 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|199|894|2165|201|715|3013; Standard Deviation|11.54|52.74|134.23|12.86|47.19|213.92; CV(%)|5.8|5.9|6.2|6.4|6.6|7.1
• Sample Data: Concentration(pg/ml)|0|125|250|500|1000|2000|4000|8000; O.D.|0.004|0.102|0.175|0.333|0.559|1.021|1.672|2.054
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Human CD97 PicoKine ELISA Kit standard curve )

Related Product Information for ADGRE5 elisa kit

Principle of the assay: Human CD97 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD97 has been precoated onto 96-well plates. Standards (NSO, Q21-Q398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD97 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD97 amount of sample captured in plate.

Background: Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 68508955
• NCBI GeneID: 976
• NCBI Accession #: NP_001020331.1
• NCBI GenBank Nucleotide #: NM_001025160.2
• UniProt Accession #: P48960; O00718; O76101; Q8NG72; Q8TBQ7; A8K7Z4; B2RBJ9
• Molecular Weight: 86,628 Da
• NCBI Official Full Name: CD97 antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor E5
• NCBI Official Symbol: ADGRE5
• NCBI Official Synonym Symbols: CD97; TM7LN1
• NCBI Protein Information: CD97 antigen
• UniProt Protein Name: CD97 antigen
• UniProt Gene Name: CD97
• UniProt Entry Name: CD97_HUMAN

NCBI Description

This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]

Uniprot Description

CD97: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration. Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; GPCR, family 2; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: extracellular space; focal adhesion; integral to plasma membrane; membrane; plasma membrane

Molecular Function: calcium ion binding; G-protein coupled receptor activity; protein binding; transmembrane receptor activity

Biological Process: cell adhesion; cell motility; cell surface receptor linked signal transduction; cell-cell signaling; G-protein coupled receptor protein signaling pathway; immune response; inflammatory response