Mouse anti-Human CD94 Monoclonal Antibody | anti-CD94 antibody

Anti-Hu CD94 Alexa Fluor700

• Gene Names: KLRD1; CD94
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD94; Monoclonal Antibody; Anti-Hu CD94 Alexa Fluor700; KLR-D1; KLRD1; KCLLRD1; killer cell lectin like receptor D1; anti-CD94 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Clone Number: HP-3D9
• Specificity: The mouse monoclonal antibody HP-3D9 recognizes an extracellular epitope of CD94, a 70kDa type II transmembrane glycoprotein expressed on NK cells, NK-T cells, and subsets of CD8+ T cells and gamma/delta T cells.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD94 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor700
• Immunogen: Cultured human NK cells
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD94 (HP-3D9) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Flow Cytometry (FC/FACS)

(Separation of human CD94 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD94 (HP-3D9) Alexa Fluor 700 antibody (4 ul reagent / 100 ul of peripheral whole blood).)

Related Product Information for anti-CD94 antibody

CD94, also known as KLRD1 (killer cell lectin-like receptor D1), is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting Functional Application (FUNC)tionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process.

References

*Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA: CD94 surface density identifies a Functional Application (FUNC)tional intermediary between the CD56bright and CD56dim human NK-cell subsets. Blood. 2010 Jan 14;115(2):274-81, URL: http://www.ncbi.nlm.nih.gov/pubmed/19897577,*Phillips JH, Chang C, Mattson J, Gumperz JE, Parham P, Lanier LL: CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA-A, HLA-B, and HLA-C allotypes. Immunity. 1996 Aug;5(2):163-72., URL: http://www.ncbi.nlm.nih.gov/pubmed/8769479,*Seo N, Tokura Y, Ishihara S, Takeoka Y, Tagawa S, Takigawa M: Disordered expression of inhibitory receptors on the NK1-type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites. Clin Exp Immunol. 2000 Jun;120(3):413-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/10844517,*Hallermalm K, Seki K, De Geer A, Motyka B, Bleackley RC, Jager MJ, Froelich CJ, Kiessling R, Levitsky V, Levitskaya J: Modulation of the tumor cell phenotype by IFN-gamma results in resistance of uveal melanoma cells to granule-mediated lysis by cytotoxic lymphocytes. J Immunol. 2008 Mar 15;180(6):3766-74., URL: http://www.ncbi.nlm.nih.gov/pubmed/18322182,*Bovenschen HJ, Van De Kerkhof PC, Gerritsen WJ, Seyger MM: The role of lesional T cells in recalcitrant psoriasis during infliximab therapy. Eur J Dermatol. 2005 Nov-Dec;15(6):454-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/16280298,*Ntrivalas EI, Kwak-Kim JY, Gilman-Sachs A, Chung-Bang H, Ng SC, Beaman KD, Mantouvalos HP, Beer AE: Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology. Hum Reprod. 2001 May;16(5):855-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/11331628,*Romero P, Ortega C, Palma A, Molina IJ, Pena J, Santamaría M: Expression of CD94 and NKG2 molecules on human CD4(+) T cells in response to CD3-mediated stimulation. J Leukoc Biol. 2001 Aug;70(2):219-24., URL: http://www.ncbi.nlm.nih.gov/pubmed/11493613,*Wada H, Matsumoto N, Maenaka K, Suzuki K, Yamamoto K: The inhibitory NK cell receptor CD94/NKG2A and the activating receptor CD94/NKG2C bind the top of HLA-E through mostly shared but partly distinct sets of HLA-E residues. Eur J Immunol. 2004 Jan;34(1):81-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/14971033

NCBI and Uniprot Product Information

• NCBI GI #: 334302835
• NCBI GeneID: 3824
• UniProt Accession #: Q13241; O43321; O43773; Q9UBE3; Q9UEQ0
• Molecular Weight: 179
• NCBI Official Full Name: Natural killer cells antigen CD94
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily D, member 1
• NCBI Official Symbol: KLRD1
• NCBI Official Synonym Symbols: CD94
• NCBI Protein Information: natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor
• UniProt Protein Name: Natural killer cells antigen CD94
• UniProt Gene Name: KLRD1
• UniProt Synonym Gene Names: CD94
• UniProt Entry Name: KLRD1_HUMAN

NCBI Description

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRD1: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to membrane; plasma membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; natural killer cell mediated immunity; innate immune response