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Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD94)

Mouse CD94 Monoclonal Antibody | anti-CD94 antibody

MOUSE ANTI HUMAN CD94

Gene Names
KLRD1; CD94
Applications
Flow Cytometry, Functional Assay
Synonyms
CD94; Monoclonal Antibody; MOUSE ANTI HUMAN CD94; anti-CD94 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
DX22
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
179
Applicable Applications for anti-CD94 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Immunogen
Natural killer cell line
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD94)

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD94)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD94 Biotin (MBS210425))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD94 Biotin (MBS210425))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD94:FITC (MBS210903))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD94:FITC (MBS210903))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD94:RPE (MBS213762))

Testing Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD94:RPE (MBS213762))
Related Product Information for anti-CD94 antibody
Mouse anti Human CD94 antibody, clone DX22 recognizes human CD94, also known as KLRD1 (Killer cell lectin-like receptor, subfamily D, member 1). CD94 is expressed on natural killer (NK) cells and a subset of T lymphocytes. CD94 is found to associate with NKG2 to form a heterodimer which is involved in the inhibition of cell mediated cytotoxicity against cells bearing appropriate MHC class I allotypes. Mouse anti Human CD94 antibody, clone DX22 is reported to inhibit the binding of CD94 to HLA-E (Braud et al. 1998) and HLA-G (Söderström. et al. 1997)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,109 Da
NCBI Official Full Name
natural killer cells antigen CD94 isoform 1
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily D, member 1
NCBI Official Symbol
KLRD1
NCBI Official Synonym Symbols
CD94
NCBI Protein Information
natural killer cells antigen CD94; CD94 antigen; KP43; NK cell receptor
UniProt Protein Name
Natural killer cells antigen CD94
UniProt Gene Name
KLRD1
UniProt Synonym Gene Names
CD94
UniProt Entry Name
KLRD1_HUMAN

NCBI Description

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRD1: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; natural killer cell mediated immunity; innate immune response

Research Articles on CD94

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Product Notes

The CD94 klrd1 (Catalog #AAA212615) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD94 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD94 klrd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD94, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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