Mouse anti-Human CD94 Monoclonal Antibody | anti-CD94 antibody

anti-human CD94-Biotin

Cat. Num. AAA666086

• Gene Names: KLRD1; CD94
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD94; Monoclonal Antibody; anti-human CD94-Biotin; Monoclonal anti-human CD94 (Kp43)/Biotin; anti-CD94 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: HP-3D9
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 54

• Applicable Applications for anti-CD94 antibody: Flow Cytometry (FC/FACS)
• Performance: Ficoll prepared human peripheral blood mononuclear cells were stimulated by incubating 1 day at 2 x 106 cells/ml in RPMI 10% FBS media including 5 mug/ml Phytohemagglutinin-P. Five x 105 cells per tube were then washed and pre incubated 10 minutes with 20 mul human IgG at 250 mug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD94/Biotin at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. A net 20% sub population of the cells stained positive with a mean shift of 1.25 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD94 antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Cultured human NK cells
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD94 antibody

Information: Human CD94 expression is restricted to natural killer (NK) cells and a small subset of T lymphocytes. CD94 is a type II membrane glycoprotein of the c-type lectin superfamily and essentially lacks a cytoplasmic tail. Antibody HP-3D9 recognizes the CD94 molecule of 70 kd. Antibody HP-3D9 inhibits IL-2 dependent proliferation of NK cells.

References

J. Aramburu, et al, (1990) J Immunol 144: 3238-3247. J. Aramburu, et al, J Immunol (1991) 147: 714-721. A. Moretta, et al, J Exp Med (1994) 180: 545-555. C. Chang, et al, Eur J Immunol (1995) 25: 2433-2437. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p.1437-1439.

NCBI and Uniprot Product Information

• NCBI GI #: 2995204
• NCBI GeneID: 3824
• UniProt Accession #: Q13241; O43321; O43773; Q9UBE3; Q9UEQ0
• Molecular Weight: 17,109 Da
• NCBI Official Full Name: CD94, partial
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily D, member 1
• NCBI Official Symbol: KLRD1
• NCBI Official Synonym Symbols: CD94
• NCBI Protein Information: natural killer cells antigen CD94; KP43; CD94 antigen; NK cell receptor
• UniProt Protein Name: Natural killer cells antigen CD94
• UniProt Gene Name: KLRD1
• UniProt Synonym Gene Names: CD94
• UniProt Entry Name: KLRD1_HUMAN

NCBI Description

Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLRD1: Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: plasma membrane; integral to membrane; receptor complex; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: regulation of immune response; cell surface receptor linked signal transduction; natural killer cell mediated immunity; innate immune response