Rat anti-Mouse CD90/Thy-1 Monoclonal Antibody | anti-THY1 antibody
Rat Anti-Mouse CD90/Thy-1-UNLB
Gene Names
THY1; CD90
Reactivity
Mouse
Applications
Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Synonyms
CD90/Thy-1; Monoclonal Antibody; Rat Anti-Mouse CD90/Thy-1-UNLB; Rat Anti-Mouse CD90/Thy-1 - Purified; Unlabeled Antibody; Rat Anti-Mouse CD90/Thy-1; Thy-1; anti-THY1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Lewis) IgG 2cK
Clone Number
G7
Specificity
Mouse CD90 (Thy-1.1 and Thy1.2 alloantigens/CD90.1 and CD90.2)
Form/Format
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Sequence Length
161
Applicable Applications for anti-THY1 antibody
Flow Cytometry; Immunocytochemistry; Immunohistochemistry (Acetone Fixed/Frozen Sections)
Application Notes
FC- Quality Tested 1,6-8
IHC-PS-Reported in literature 4
IP- Reported in literature 1
Stim-Reported in literature 1
Activ-Reported in literature 1,5
Apop-Reported in literature 2, 3
Working Dilutions:
Flow Cytometry
FITC and BIOT conjugates <= 1 ug/106 cells.
SPRD and APC conjugates <=0.2 ug/ 10 6 cells.
PE conjugate <=0.1 ug/ 106 cells.
For flow cytometry, the suggested use of these reagents is in final volume of 100 uL.
Other Applications:
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
IHC-PS-Reported in literature 4
IP- Reported in literature 1
Stim-Reported in literature 1
Activ-Reported in literature 1,5
Apop-Reported in literature 2, 3
Working Dilutions:
Flow Cytometry
FITC and BIOT conjugates <= 1 ug/106 cells.
SPRD and APC conjugates <=0.2 ug/ 10 6 cells.
PE conjugate <=0.1 ug/ 106 cells.
For flow cytometry, the suggested use of these reagents is in final volume of 100 uL.
Other Applications:
Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need.
Immunogen
Mouse T cell hybridoma C6/G8
Preparation and Storage
Store at 2-8°C.
Related Product Information for anti-THY1 antibody
CD90 (Thy-1), a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily of cell surface receptors, consists of a single V-set domain. It is expressed on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes, and neurons of all mouse strains. The monoclonal antibody G7 stimulates T-cell proliferation and IL-2 secretion via signaling through the T-cell receptor/CD3 complex. G7 has also been reported to promote apoptosis of thymocytes and CTL clones and to mediate adhesion of thymocytes to thymic stroma.
References
1. Gunter, K., T. Malek, and E. Shevach. 1984. T-cell activating properties of an anti-Thy-1 monoclonal antibody. J. Exp. Med. 159:716.
2. Williams, A.F., and J. Gagnon. 1982. Science 216:696.
3. Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Nature 322:181.
4. Hueber, A.O., G. Raposo, M. Pierres, and H.-T. He. 1994. J. Exp. Med. 179:785.
5. Tentori, L., D.M. Pardoll, J.C. Zunigaq, J. Hu-Li, W.E. Paul, J.A. Bluestone, and A.M. Kruisbeek. 1988. J. Immunol. 140:1089.
6. Sugiyama, E., A. Cantagrei, T. Reno, F. Stafford-Brady, E.T.H. Yeh, and J.V. Bonventre. 1990. Cell. Immunol. 130:271.
7. Ucker, D.S., J. Meyers, and P.S. Obermuller. 1992. J. Immunol. 149:1583.
8. He, H.-T., P. Naquet, D. Caillol, and M. Pierres. 1991. J. Exp. Med. 173:515.
9. Kruisbeek, A.M., and E. Shevach. 1991. Proliferative assays for T cell function. In: Current Protocols in Immunology. Coligan, J., A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober. John Wiley & Sons, New York, NY, pp. 3.12.1-3.12.14.
2. Williams, A.F., and J. Gagnon. 1982. Science 216:696.
3. Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Nature 322:181.
4. Hueber, A.O., G. Raposo, M. Pierres, and H.-T. He. 1994. J. Exp. Med. 179:785.
5. Tentori, L., D.M. Pardoll, J.C. Zunigaq, J. Hu-Li, W.E. Paul, J.A. Bluestone, and A.M. Kruisbeek. 1988. J. Immunol. 140:1089.
6. Sugiyama, E., A. Cantagrei, T. Reno, F. Stafford-Brady, E.T.H. Yeh, and J.V. Bonventre. 1990. Cell. Immunol. 130:271.
7. Ucker, D.S., J. Meyers, and P.S. Obermuller. 1992. J. Immunol. 149:1583.
8. He, H.-T., P. Naquet, D. Caillol, and M. Pierres. 1991. J. Exp. Med. 173:515.
9. Kruisbeek, A.M., and E. Shevach. 1991. Proliferative assays for T cell function. In: Current Protocols in Immunology. Coligan, J., A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober. John Wiley & Sons, New York, NY, pp. 3.12.1-3.12.14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
CD90 protein
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90
NCBI Protein Information
thy-1 membrane glycoprotein
UniProt Protein Name
CD90 protein
Protein Family
UniProt Gene Name
CD90
UniProt Entry Name
B9ZSM8_PIG
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Product Notes
The THY1 cd90 (Catalog #AAA673460) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD90/Thy-1-UNLB reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD90/Thy-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunocytochemistry; Immunohistochemistry (Acetone Fixed/Frozen Sections). FC- Quality Tested 1,6-8 IHC-PS-Reported in literature 4 IP- Reported in literature 1 Stim-Reported in literature 1 Activ-Reported in literature 1,5 Apop-Reported in literature 2, 3 Working Dilutions: Flow Cytometry FITC and BIOT conjugates <= 1 ug/106 cells. SPRD and APC conjugates <=0.2 ug/ 10 6 cells. PE conjugate <=0.1 ug/ 106 cells. For flow cytometry, the suggested use of these reagents is in final volume of 100 uL. Other Applications: Since applications vary, you should determine the optimum working dilution for the product that is appropriate for your specific need. Researchers should empirically determine the suitability of the THY1 cd90 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90/Thy-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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