Rabbit OFD1 Polyclonal Antibody | anti-OFD1 antibody

OFD1 Antibody

Cat. Num. AAA9611957

• Gene Names: OFD1; RP23; 71-7A; SGBS2; CXorf5; JBTS10
• Reactivity: Human; Predicted: Bovine, Horse, Dog
• Applications: Western Blot
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: OFD1; Polyclonal Antibody; OFD1 Antibody; 71 7A; 717A; CXorf5; JBTS10; Ofd1; OFD1_HUMAN; oral facial digital syndrome 1; Oral-facial-digital syndrome 1 protein; OTTHUMP00000022941; Protein 71-7A; RGD1562231; SGBS2; anti-OFD1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Bovine, Horse, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OFD1 Antibody detects endogenous levels of OFD1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1012

• Applicable Applications for anti-OFD1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human OFD1.
• Tissue Specificity: Widely expressed. Expressed in 9 and 14 weeks old embryos in metanephric mesenchyme, oral mucosa, lung, heart, nasal and cranial cartilage, and brain. Expressed in metanephros, brain, tongue, and limb.
• Fragment: Fab Fragment
• Conjugate: Unconjugated
• Subcellular Location: Nucleus; Cytoskeleton
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using OFD1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-OFD1 antibody

Description: Component of the centrioles controlling mother and daughter centrioles length. Recruits to the centriole IFT88 and centriole distal appendage-specific proteins including CEP164. Involved in the biogenesis of the cilium, a centriole-associated function. The cilium is a cell surface projection found in many vertebrate cells required to transduce signals important for development and tissue homeostasis. Plays an important role in development by regulating Wnt signaling and the specification of the left-right axis. Only OFD1 localized at the centriolar satellites is removed by autophagy, which is an important step in the ciliogenesis regulation (By similarity).

Subunit Structure: Homooligomer. Interacts with LCA5. Interacts with RUVBL1; the interaction is direct and may mediate interaction with the NuA4 histone acetyltransferase complex. Interacts with SDCCAG8; the interaction is direct. Interacts with MAP1LC3B. Interacts with C2CD3; OFD1 may act as a egative regulator of C2CD3. Forms a complex with KIAA0753/OFIP and FOPNL/FOR20; the interaction with FOPNL is detected only in the presence of KIAA0753. Interacts with PCM1; this interaction may be mediated by KIAA0753/OFIP (PubMed:26643951).

Similarity: Belongs to the OFD1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 4503179
• NCBI GeneID: 8481
• NCBI Accession #: NP_003602
• NCBI GenBank Nucleotide #: NP_003602.1
• UniProt Accession #: O75665
• Molecular Weight: Observed: 43 kDa; Predicted: 117 kDa
• NCBI Official Full Name: oral-facial-digital syndrome 1 protein isoform 1
• NCBI Official Synonym Full Names: OFD1 centriole and centriolar satellite protein
• NCBI Official Symbol: OFD1
• NCBI Official Synonym Symbols: RP23; 71-7A; SGBS2; CXorf5; JBTS10
• NCBI Protein Information: oral-facial-digital syndrome 1 protein
• UniProt Protein Name: Oral-facial-digital syndrome 1 protein
• Protein Family: Oral-facial-digital syndrome 1 protein
• UniProt Gene Name: OFD1
• UniProt Synonym Gene Names: CXorf5
• UniProt Entry Name: OFD1_HUMAN

NCBI Description

This gene is located on the X chromosome and encodes a centrosomal protein. A knockout mouse model has been used to study the effect of mutations in this gene. The mouse gene is also located on the X chromosome, however, unlike the human gene it is not subject to X inactivation. Mutations in this gene are associated with oral-facial-digital syndrome type I and Simpson-Golabi-Behmel syndrome type 2. Many pseudogenes have been identified; a single pseudogene is found on chromosome 5 while as many as fifteen have been found on the Y chromosome. [provided by RefSeq, Aug 2016]