Rabbit anti-Human VAV1 Polyclonal Antibody | anti-VAV1 antibody

Anti-VAV1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8101253

• Gene Names: VAV1; VAV
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: VAV1; Polyclonal Antibody; Anti-VAV1 Antibody; Rabbit Polyclonal; VAV1 Antibody; Rabbit PAb; Antigen Affinity Purified; vav guanine nucleotide exchange factor 1; Anti-VAV Antibody; anti-VAV1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human VAV1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-VAV1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human VAV1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human VAV1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-VAV1 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: MOLT-4 Whole Cell LysateLane C: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:98 kDaObserved band size:90 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-VAV1 antibody

VAV1, short for vav guanine nucleotide exchange factor 1, is normally expressed exclusively in the hematopoietic system, where it functions as a specific GDP/GTP nucleotide exchange factor (GEF), strictly regulated by tyrosine phosphorylation. Vav1 contains many characteristic structural motifs important for its function as a versatile signal transducer. In hematopoietic cells, Vav1 is phosphorylated following cell surface receptor activation, triggering re-organization of the cytoskeleton and regulation of other cellular functions including transcription, cytokine production, cell cycle progression, and Ca(2+) mobilization.

Product Categories/Family for anti-VAV1 antibody

Actin Dynamics Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 7108367
• NCBI GeneID: 7409
• NCBI Accession #: NP_005419.2
• NCBI GenBank Nucleotide #: NM_005428.3
• UniProt Accession #: P15498; Q15860
• Molecular Weight: 98,314 Da
• NCBI Official Full Name: proto-oncogene vav isoform 1
• NCBI Official Synonym Full Names: vav 1 guanine nucleotide exchange factor
• NCBI Official Symbol: VAV1
• NCBI Official Synonym Symbols: VAV
• NCBI Protein Information: proto-oncogene vav; vav 1 oncogene
• UniProt Protein Name: Proto-oncogene vav
• Protein Family: Proto-oncogene
• UniProt Gene Name: VAV1
• UniProt Synonym Gene Names: VAV
• UniProt Entry Name: VAV_HUMAN

NCBI Description

This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Function: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.

Subunit structure: May interact with CCPG1

By similarity. Interacts with APS, DOCK2, GRB2, GRB3, DOCK2, SLA, TEC and ZNF655/VIK. Interacts with SIAH2; without leading to its degradation. Associates with BLNK, PLCG1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB; which inhibits tyrosine phosphorylation and down-regulates activity. Interacts with SHB and CLNK

By similarity. Interacts with THEMIS2. Interacts with HCK

By similarity. Interacts with NEK3 and this interaction is prolactin-dependent. Interacts with ITK. Interacts with PTK2B/PYK2. Interacts (via SH2 domain) with SYK. Interacts with ANKRD54

By similarity. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17 Ref.19 Ref.20

Tissue specificity: Widely expressed in hematopoietic cells but not in other cell types.

Domain: The DH domain is involved in interaction with CCPG1

By similarity.

Post-translational modification: Phosphorylated on tyrosine residues by HCK in response to IFNG and bacterial lipopolysaccharide (LPS)

By similarity. Phosphorylated by FYN. Ref.12

Miscellaneous: 'Vav' stands for the sixth letter of the Hebrew alphabet.

Sequence similarities: Contains 1 CH (calponin-homology) domain.Contains 1 DH (DBL-homology) domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 SH2 domain.Contains 2 SH3 domains.

Sequence caution: The sequence CAA34383.1 differs from that shown. Reason: Frameshift at positions 322 and 355.