Mouse anti-Rat CD8b Monoclonal Antibody | anti-CD8b antibody

Anti-Rat CD8b Biotin (Clone 3.4.1) (mouse IgG1)

Cat. Num. AAA520234

• Gene Names: CD8B; LY3; P37; LEU2; LYT3; CD8B1
• Reactivity: Rat
• Applications: Immunohistochemistry
• Synonyms: CD8b; Monoclonal Antibody; Anti-Rat CD8b Biotin (Clone 3.4.1) (mouse IgG1); CD8b Biotin (Clone 3.4.1) (mouse IgG1); Biotin Anti-Rat CD8b Monoclonal Antibody; anti-CD8b antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: mouse IgG1,K
• Clone Number: 3.4.1
• Specificity: CD8b (á chain) (Rat CD8b)
• Form/Format: Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography).)
• Concentration: Antibody Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 243

• Applicable Applications for anti-CD8b antibody: Immunohistochemistry (IHC)
• Immunogen: Rat/mouse T cell hybrids expressing CD8
• Donor: BALB/c mouse spleen cells
• Fusion Partner: X63 Ag.653 myeloma cell line
• Presentation: 100ug or 500 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabalizing protein to bring total protein concentration to 4-5 mg/ml.
• Preparation and Storage: Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles. Avoid prolonged exposure to light.

Testing Data

Related Product Information for anti-CD8b antibody

anti-rat CD8b monoclonal antibody reacts with the beta chain of the CD8 differentiation antigen. CD8b is expressed on most thymocytes and mature T cytotoxic/suppressor cells (MHC class I restricted). While the CD8a and CD8b form a heterodimer on the surface of thymocytes and thymus-dependent T cytotoxic/suppressor cells, the majority of NK cells, many CD8 T cells from athymic rats, many activated CD4 T cells, and intestinal epithelium lymphocytes (IEL) express CD8a without CD8b. This suggests that expression of the CD8 heterodimer (a/b) is more dependant on intrathymic T cell maturation than that of the homodimer (a/a). The thymus dependence of CD8a/b T cells may be due to a requirement for thymic selection on self MHC class I antigens. Reported applications for this antibody include flow cytometry, immunoprecipitation and Western blotting. The 3.4.1 antibody also blocks both activation in an allogenic response and cell mediated cytotoxicity by CD8 T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 71682667
• NCBI GeneID: 926
• UniProt Accession #: P10966; P14860; P14861; Q15980; Q496E0; Q496E1; Q496E2; Q9UDB4; Q9UDB5; Q9UDB6; Q9UDB7; Q9UDB8
• Molecular Weight: 23,722 Da
• NCBI Official Full Name: CD8b molecule
• NCBI Official Synonym Full Names: CD8b molecule
• NCBI Official Symbol: CD8B
• NCBI Official Synonym Symbols: LY3; P37; LEU2; LYT3; CD8B1
• NCBI Protein Information: T-cell surface glycoprotein CD8 beta chain; CD8 antigen, beta polypeptide 1 (p37); T lymphocyte surface glycoprotein beta chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 beta chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8B
• UniProt Synonym Gene Names: CD8B1
• UniProt Entry Name: CD8B_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. [provided by RefSeq, May 2010]

Uniprot Description

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds.

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein

Probable. Isoform 2: Cell membrane; Single-pass type I membrane protein

Probable. Isoform 3: Secreted

Probable. Isoform 4: Cell membrane; Single-pass type I membrane protein

Probable. Isoform 5: Cell membrane; Single-pass type I membrane protein

Probable. Isoform 6: Secreted

Probable. Isoform 7: Secreted

Probable. Isoform 8: Secreted

Probable.

Tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. Ref.7

Post-translational modification: Phosphorylated as a consequence of T-cell activation

Potential.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence caution: The sequence AAD13877.1 differs from that shown. Reason: Erroneous gene model prediction.