Mouse anti-Rat CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Rat CD8a Purified (Clone OX-8) (mouse IgG1)

Cat. Num. AAA520227

• Gene Names: CD8A; CD8; MAL; p32; Leu2
• Reactivity: Rat
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assay
• Synonyms: CD8a; Monoclonal Antibody; Anti-Rat CD8a Purified (Clone OX-8) (mouse IgG1); CD8a Purified (Clone OX-8) (mouse IgG1); Purified Anti-Rat CD8a Monoclonal Antibody; anti-CD8a antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: OX-8
• Specificity: CD8a (Rat CD8a)
• Form/Format: Purified
• Sequence Length: 235

• Applicable Applications for anti-CD8a antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), Flow Cytometry (FC/FACS)
• Immunogen: High molecular weight rat thymocyte glycoproteins
• Donor: BALB/c spleen
• Fusion Partner: P3-NSI-1-Ag4 (NS1/1)
• Presentation: 250ug or 500ug Purified Ig buffered in PBS and 0.02% NaN3.
• Preparation and Storage: Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze thaw cycles.

Testing Data

Related Product Information for anti-CD8a antibody

anti-rat T cytotoxic/suppressor cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine (1,2,3,4). The antigen recognized is a complex of surface glycoproteins of Mr 34, 39, and 76 kDa and is the rat homologue of the human CD8 and the mouse Ly 2.3 antigen (5,8). MBS520227 labels all peripheral T cells that are unlabelled by the (W3/25) monoclonal antibody. It labels a T cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor (2). MBS520227 and can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft versus host disease (7). This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being (W3/13) which labels all T cells, as well as (W3/25) and MBS520227 (MRC OX-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).

NCBI and Uniprot Product Information

• NCBI GI #: 19344022
• NCBI GeneID: 925
• UniProt Accession #: P01732; Q13970; Q4ZG17; D6W5M8
• Molecular Weight: 25,729 Da
• NCBI Official Full Name: CD8a molecule
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; MAL; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL
• UniProt Entry Name: CD8A_HUMAN

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Subunit structure: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner. Ref.9

Subcellular location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Post-translational modification: All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not

By similarity.

Involvement in disease: Familial CD8 deficiency (CD8 deficiency) [MIM:608957]: Novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.