Mouse anti-Human Erythropoietin (EPO) Antibody | anti-EPO antibody

Erythropoietin (EPO) (Marker of Placentation Disorders)

Cat. Num. AAA4380859

• Gene Names: EPO; EP; DBAL; ECYT5; MVCD2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Erythropoietin (EPO); Erythropoietin (EPO) (Marker of Placentation Disorders); EP; EPO alpha; EPO; Epoetin; Erythropoietin; MVCD2; anti-EPO antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rEPO/1367
• Specificity: Recognizes a protein of about 37kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 193

• Applicable Applications for anti-EPO antibody: ELISA (EIA); Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating (Order Ab without BSA); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HepG2 cells. Heart or Kidney.
• Immunogen: Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasmic and Cell Surface
• Hu-chromosome Location: 7q22
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified EPO Recombinant Mouse Monoclonal Antibody (rEPO/1367). Confirmation of Purity and Integrity of Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human kidney stained with EPO Recombinant Mouse Monoclonal Antibody (rEPO/1367).)

References

Jelkmann, W. 1992. Erythropoietin: structure, control of production, and function. Physiol. Rev. 72: 449

NCBI and Uniprot Product Information

• NCBI GI #: 62240997
• NCBI GeneID: 2056
• NCBI Accession #: NP_000790.2
• NCBI GenBank Nucleotide #: NP_000790.2
• UniProt Accession #: P01588
• Molecular Weight: 37kDa
• NCBI Official Full Name: erythropoietin
• NCBI Official Synonym Full Names: erythropoietin
• NCBI Official Symbol: EPO
• NCBI Official Synonym Symbols: EP; DBAL; ECYT5; MVCD2
• NCBI Protein Information: erythropoietin
• UniProt Protein Name: Erythropoietin
• Protein Family: Erythropoietin
• UniProt Gene Name: EPO
• UniProt Entry Name: EPO_HUMAN

NCBI Description

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Uniprot Description

EPO: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Genetic variation in EPO is associated with susceptbility to microvascular complications of diabetes type 2 (MVCD2). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Belongs to the EPO/TPO family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: cell surface; extracellular region; extracellular space

Molecular Function: protein binding

Biological Process: erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of Ras protein signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein

Disease: Microvascular Complications Of Diabetes, Susceptibility To, 2