Mouse anti-Ferret CD8 alpha Monoclonal Antibody | anti-CD8A antibody

Anti-CD8 alpha Antibody, Mouse Monoclonal

Cat. Num. AAA8112442

• Reactivity: Ferret
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: CD8 alpha; Monoclonal Antibody; Anti-CD8 alpha Antibody; Mouse Monoclonal; CD8a Antibody; Mouse MAb; CD8a molecule; anti-CD8A antibody

• Host: Mouse
• Reactivity: Ferret
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 3B5H2
• Specificity: Ferret CD8 alpha.; No cross-reactivity in ELISA with Human CD8A, Mouse CD8A, Human cell lysate (293 cell line)
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD8A antibody: ELISA (EIA), ELISA (EIA) (Det)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Det): 1:1000-1:10,000; ELISA (Det): This antibody will detect Ferret CD8/CD8 alpha/Leu-2 in ELISA pair set.; In a sandwich ELISA, it can be used as detection antibody when paired with.
• Immunogen: Recombinant Ferret CD8a protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD8a (rF CD8a). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CD8A antibody

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B-and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.

References

References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

NCBI and Uniprot Product Information

• Protein Family: T-cell surface glycoprotein