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Western Blot (WB) (Western Blot analysis of ORC6L expression in transfected 293T cell line by ORC6L polyclonal antibody. Lane 1: ORC6L transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

Rabbit anti-Human ORC6L Polyclonal Antibody | anti-ORC6L antibody

ORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (HRP)

Gene Names
ORC6; ORC6L
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
ORC6L; Polyclonal Antibody; ORC6L (Origin Recognition Complex Subunit 6-like; Origin Recognition Complex Subunit 6 Homolog-like (yeast); Origin Recognition Complex Subunit 6 Homolog-like 2; Origin Recognition Complex Subunit 6; ORC6) (HRP); anti-ORC6L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ORC6L.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Sequence Length
1615
Applicable Applications for anti-ORC6L antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human ORC6L, aa1-252 (NP_055136.1).
Immunogen Sequence
MGSELIGRLAPRLGLAEPDMLRKAEEYLRLSRVKCVGLSARTTETSSAVMCLDLAASWMKCPLDRAYLIKLSGLNKETYQSCLKSFECLLGLNSNIGIRDLAVQFSCIEAVNMASKILKSYESSLPQTQQVDLDLSRPLFTSAALLSACKILKLKVDKNKMVATSGVKKAIFDRLCKQLEKIGQQVDREPGDVATPPRKRKKIVVEAPAKEMEKVEEMPHKPQKDEDLTQDYEEWKRKILENAASAQKATAE
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ORC6L expression in transfected 293T cell line by ORC6L polyclonal antibody. Lane 1: ORC6L transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of ORC6L expression in transfected 293T cell line by ORC6L polyclonal antibody. Lane 1: ORC6L transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-ORC6L antibody
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L,-3L,-4L and-5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis.
Product Categories/Family for anti-ORC6L antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Homo sapiens origin recognition complex subunit 6 (ORC6), transcript variant 1, mRNA
NCBI Official Synonym Full Names
origin recognition complex subunit 6
NCBI Official Symbol
ORC6
NCBI Official Synonym Symbols
ORC6L
NCBI Protein Information
origin recognition complex subunit 6
UniProt Protein Name
Origin recognition complex subunit 6
UniProt Gene Name
ORC6
UniProt Synonym Gene Names
ORC6L
UniProt Entry Name
ORC6_HUMAN

NCBI Description

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq, Oct 2010]

Uniprot Description

ORC6L: Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. ORC is composed of six subunits. In human, ORC is cell cycle-dependent regulated: it is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Belongs to the ORC6 family.

Protein type: DNA replication

Chromosomal Location of Human Ortholog: 16q12

Cellular Component: nucleoplasm; membrane; plasma membrane; nuclear origin of replication recognition complex; nucleus; origin recognition complex

Molecular Function: protein binding; DNA binding

Biological Process: mitotic cell cycle; DNA replication; DNA-dependent DNA replication; G1/S transition of mitotic cell cycle

Disease: Meier-gorlin Syndrome 3

Research Articles on ORC6L

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Product Notes

The ORC6L orc6 (Catalog #AAA6388078) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORC6L (Origin Recognition Complex Subunit 6-like, Origin Recognition Complex Subunit 6 Homolog-like (yeast), Origin Recognition Complex Subunit 6 Homolog-like 2, Origin Recognition Complex Subunit 6, ORC6) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORC6L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ORC6L orc6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORC6L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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