Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (MBS488750) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version HM79-16 (MBS488751) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488751, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
anti-Mouse CD79b Monoclonal Antibody | anti-CD79b antibody
Anti-CD79b [HM79-16]
Gene Names
Cd79b; B29; Igb; Igbeta; Ig-beta
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD79b; Monoclonal Antibody; Anti-CD79b [HM79-16]; Ig-beta; AGM6; B29; IGB; CD79b molecule;; anti-CD79b antibody
Host
Hamster (Armenian)
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster (Armenian) IgG, lambda
Clone Number
HM79-16
Specificity
Reacts with an extracellular epitope of CD79b.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD79b antibody
Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008).
PMID
9418137
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (MBS488750) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version HM79-16 (MBS488751) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488751, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (MBS488750) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version HM79-16 (MBS488751) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488751, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Western Blot (WB)
(Western Blot using anti-CD79b antibody HM79-16 (MBS488750) Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HM79-16 (MBS488751) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD79b antibody HM79-16 (MBS488750) Mouse spleen tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HM79-16 (MBS488751) at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (MBS488750) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version HM79-16 (MBS488751) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488751, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD79b antibody HM79-16 (MBS488750) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version HM79-16 (MBS488751) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488751, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD79b antibody
Recombinant monoclonal antibody to CD79b. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HM79-16.
HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immuno-precipitationRecombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Product Categories/Family for anti-CD79b antibody
References
Mariko Koyama et al. CD79alpha/CD79beta heterodimers are expressed on pro-B cell surfaces without associated u heavy chain International Immunology, Vol. 9, No. 11, pp. 1767-1772 1997
Note on publication: Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.
Note on publication: Describes the expression of the CD79b and the use of new monoclonal antibodes to detect CD79b on pro-B cell surfaces.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,726 Da
NCBI Official Full Name
B-cell antigen receptor complex-associated protein beta chain
NCBI Official Synonym Full Names
CD79B antigen
NCBI Official Symbol
Cd79b
NCBI Official Synonym Symbols
B29; Igb; Igbeta; Ig-beta
NCBI Protein Information
B-cell antigen receptor complex-associated protein beta chain; immunoglobulin-associated beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein
UniProt Protein Name
B-cell antigen receptor complex-associated protein beta chain
Protein Family
UniProt Gene Name
Cd79b
UniProt Synonym Gene Names
Igb
UniProt Entry Name
CD79B_MOUSE
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Product Notes
The CD79b cd79b (Catalog #AAA488750) is an Antibody produced from Hamster (Armenian) and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD79b [HM79-16] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD79b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC). A biotinylated HM79-16 was used and stained with streptavidin-FITC for analysis by FACS to identify variants of B lymphoma cell lines that have lost expression of the mu heavy chain, and for staining using a biotinylated HM79-16 (Condon 2000). HM79-16 has also been used in competitive binding assays to study its effect on the binding of CD79a to CD79b in the context of ameliorating autoimmune disease in mice (Yongmei Li 2008). Researchers should empirically determine the suitability of the CD79b cd79b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD79b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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