Mouse anti-Mouse CD72.1 (Lyb-2.1) Monoclonal Antibody
Mouse Anti-Mouse CD72.1-BIOT
Reactivity
Mouse
Applications
Flow Cytometry
Synonyms
CD72.1 (Lyb-2.1); Monoclonal Antibody; Mouse Anti-Mouse CD72.1-BIOT; Mouse Anti-Mouse CD72.1 - Biotin; Mouse Anti-Mouse CD72.1 (Lyb-2.1); anti-CD72.1 (Lyb-2.1) antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse (C57/BL6) IgG2b kappa
Clone Number
10-1.D.2
Specificity
Mouse CD72.1/Lyb-2.1, 45 kDa
Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells.^1,2 The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.^1 However, 10-1.D.2 can itself trigger a positive signal by binding CD72.^1-5
Murine CD72.1/Lyb-2.1, a type II integral membrane glycoprotein and a member of the C-lectin family of cell surface receptors, is a differentiation antigen of B cells, and is found in mouse strains expressing the Lyb-2.1 allotype. CD72.1 is the ligand of CD5 (Ly-1) which is distributed on all T cells and a small number of B cells.^1,2 The 10-1.D.2 monoclonal antibody blocks binding of CD5 to CD72 on the B cell surface, which leads to inhibition of the positive signal resulting from CD5/CD72 pairing.^1 However, 10-1.D.2 can itself trigger a positive signal by binding CD72.^1-5
Form/Format
Biotin (BIOT) Conjugate
Applicable Applications for anti-CD72.1 (Lyb-2.1) antibody
Flow Cytometry
Application Notes
Research Application(s): In vitro blocking and proliferation assays
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells
Immunogen
DBA/2 mouse spleen cells
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent. Representative flow data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
All products should be stored at 2-8 degree C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Luo, W., H Van de Velde, I. von Hoegen, J.R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630.
2. Subbarao, B., and D.E. Mosier. 1983. J. Immunol. 130:2033.
3. Snow, E.C., J.J. Mond, and B. Subbarao. 1986. J. Immunol. 137:1793.
4. Robinson, W.H., M.M Landolfi, H. Schaffer and J.R. Parnes. 1993. J. Immunol. 151:4764.
5. Beavil, A.J., R.L. Edmeades, H.J. Gould and B.J. Sutton. 1992. Proc. Nat. Acad. Sci. 89:753.
2. Subbarao, B., and D.E. Mosier. 1983. J. Immunol. 130:2033.
3. Snow, E.C., J.J. Mond, and B. Subbarao. 1986. J. Immunol. 137:1793.
4. Robinson, W.H., M.M Landolfi, H. Schaffer and J.R. Parnes. 1993. J. Immunol. 151:4764.
5. Beavil, A.J., R.L. Edmeades, H.J. Gould and B.J. Sutton. 1992. Proc. Nat. Acad. Sci. 89:753.
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Product Notes
The CD72.1 (Lyb-2.1) (Catalog #AAA673441) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse CD72.1-BIOT reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD72.1 (Lyb-2.1) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Research Application(s): In vitro blocking and proliferation assays Flow Cytometry Suggested Dilution: <= 1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD72.1 (Lyb-2.1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD72.1 (Lyb-2.1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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