Mouse anti-Human CD70 / TNFSF7 Monoclonal Antibody | anti-CD70 antibody

CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439093

• Gene Names: CD70; CD27L; CD27LG; TNFSF7; TNLG8A
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
• Synonyms: CD70 / TNFSF7; Monoclonal Antibody; CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker) Mouse Monoclonal Antibody; CD27 ligand (CD27L); CD27LG; Ki24; Surface antigen CD70; Tumor necrosis factor (ligand) superfamily; member 7 (TNFSF7); anti-CD70 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: BU69
• Specificity: It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis. This MAb blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 193

• Applicable Applications for anti-CD70 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC) Formalin
• Application Notes: ELISA (For coating, order Ab without BSA); Inhibits T cell proliferation induced by dendritic cells (Order Ab without Azide); Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunoprecipitation (0.5-1 -g/500ug protein Lysate); Immunohistochemistry (Frozen) (0.5-1ug/ml for 30 minutes at RT)
• Cellular Localization: Cell Surface
• Immunogen: Human WM-1 (Waldenstrom s macroglobulinemia) cell line
• Hu-Chromosome Location: 19p13
• Positive Control: Raji cells. Lymph node or tonsil.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

R.Q. Hintzen, et al, (1994) J Immunol 152: 1762-1773. M.R. Bowman, et al, (1994) J Immunol 152: 1756-1761. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1137-1138. K. Agematsu, et al, (1995) J Immunol 154: 3627-3635

NCBI and Uniprot Product Information

• NCBI GI #: 4507605
• NCBI GeneID: 970
• NCBI Accession #: NP_001243.1
• NCBI GenBank Nucleotide #: NM_001252.4
• UniProt Accession #: P32970; Q53XX4; Q96J57; B4DPR8
• Molecular Weight: 30kDa
• NCBI Official Full Name: CD70 antigen
• NCBI Official Synonym Full Names: CD70 molecule
• NCBI Official Symbol: CD70
• NCBI Official Synonym Symbols: CD27L; CD27LG; TNFSF7; TNLG8A
• NCBI Protein Information: CD70 antigen
• UniProt Protein Name: CD70 antigen
• Protein Family: CD70 antigen
• UniProt Gene Name: CD70
• UniProt Synonym Gene Names: CD27L; CD27LG; TNFSF7; CD27-L
• UniProt Entry Name: CD70_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis. [provided by RefSeq, Jul 2008]

Uniprot Description

CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.

Protein type: Membrane protein, integral; Cytokine

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protease binding; protein binding; receptor binding

Biological Process: cell proliferation; cell-cell signaling; signal transduction; tumor necrosis factor-mediated signaling pathway