Human Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit | SDHB elisa kit

Human Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit

Cat. Num. AAA457159

• Gene Names: SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
• Reactivity: Human
• Synonyms: Succinate Dehydrogenase Complex Subunit B (SDHB); Human Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit; SDH1; SDH; Ip; Iron Sulfur; Iron-sulfur subunit of complex II; Succinate dehydrogenase [ubiquinone] iron-sulfur subunit; mitochondrial; SDHB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SDHB. No significant cross-reactivity or interference between SDHB and analogues was observed.
• Sequence Length: 280

• Samples: Tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.067ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SDHB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SDHB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDHB elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SDHB in human tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDHB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SDHB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDHB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SDHB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 115387094
• NCBI GeneID: 6390
• NCBI Accession #: NP_002991.2
• NCBI GenBank Nucleotide #: NM_003000.2
• UniProt Accession #: P21912; Q0QEY7; Q9NQ12; B2R545
• Molecular Weight: 31,630 Da
• NCBI Official Full Name: succinate dehydrogenase
• NCBI Official Synonym Full Names: succinate dehydrogenase complex iron sulfur subunit B
• NCBI Official Symbol: SDHB
• NCBI Official Synonym Symbols: IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP
• NCBI Protein Information: succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• UniProt Protein Name: Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• Protein Family: Succinate dehydrogenase
• UniProt Gene Name: SDHB
• UniProt Synonym Gene Names: SDH; SDH1; Ip

NCBI Description

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).