CD69 recombinant protein

Recombinant Human CD69 Protein (His tag)

Cat. Num. AAA2545894

• Gene Names: CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CD69; Recombinant Human CD69 Protein (His tag); CLEC2C; CD69 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 199

• Application Notes: The secreted recombinant human CD69 comprises 149 amino acids with a predicted molecular mass of 17.4 kDa. CD69 exists as a disulfide-linked homodimeric protein and migrates as an approximately 40 kDa band in SDS-PAGE under non-reduced conditions due to glycosylation.
• Predicted N Terminal: Ser 62
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD69 recombinant protein

Background: Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator, potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.

Description: A DNA sequence encoding the human CD69 (NP_001772.1) extracellular domain (Ser 62-Lys 199) was fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4502681
• NCBI GeneID: 969
• NCBI Accession #: NP_001772.1
• NCBI GenBank Nucleotide #: NM_001781.2
• UniProt Accession #: Q07108
• Molecular Weight: 22,559 Da
• NCBI Official Full Name: early activation antigen CD69
• NCBI Official Synonym Full Names: CD69 molecule
• NCBI Official Symbol: CD69
• NCBI Official Synonym Symbols: AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
• NCBI Protein Information: early activation antigen CD69
• UniProt Protein Name: Early activation antigen CD69
• Protein Family: Early activation antigen
• UniProt Gene Name: CD69
• UniProt Synonym Gene Names: CLEC2C; AIM

NCBI Description

This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.