Rabbit anti-Human ABCB6 Polyclonal Antibody | anti-ABCB6 antibody

ABCB6 Antibody (C-term)

Cat. Num. AAA9232344

• Gene Names: ABCB6; ABC; PRP; umat; ABC14; MTABC3; FLJ22414
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Synonyms: ABCB6; Polyclonal Antibody; ABCB6 Antibody (C-term); Purified Rabbit Polyclonal Antibody (Pab); ATP-binding cassette sub-family B member 6; mitochondrial; Mitochondrial ABC transporter 3; Mt-ABC transporter 3; P-glycoprotein-related protein; Ubiquitously-expressed mammalian ABC half transporter; MTABC3; PRP; UMAT; anti-ABCB6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB1810
• Specificity: This ABCB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 812-842 amino acids from the C-terminal region of human ABCB6.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

• Applicable Applications for anti-ABCB6 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: WB: 1:1000; IHC-P: 1:50~100
• Antigen Source: Human
• Antigen Region: 812-842
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(The anti-ABCB6 C-term Pab is used in Western blot to detect ABCB6 in HL60 cell lysate.)

Immunohistochemistry (IHC)-Paraffin

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-ABCB6 antibody

The membrane-associated protein ABCB6 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This half-transporter likely plays a role in mitochondrial function. Localized to 2q26, this gene is considered a candidate gene for lethal neonatal metabolic syndrome, a disorder of mitochondrial function.

Product Categories/Family for anti-ABCB6 antibody

Primary Antibodies; Cancer; Cardiovascular; Metabolism; Signal Transduction

References

Mitsuhashi, N., et al., J. Biol. Chem. 275(23):17536-17540 (2000).Furuya, K.N., et al., Cancer Res. 57(17):3708-3716 (1997).Allikmets, R., et al., Hum. Mol. Genet. 5(10):1649-1655 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 9955963
• NCBI GeneID: 10058
• NCBI Accession #: NP_005680.1
• NCBI GenBank Nucleotide #: NM_005689.2
• UniProt Accession #: Q9NP58; O75542; Q49A66; Q59GQ5; Q6ZME6; Q96ME8; Q9HAQ6; Q9HAQ7
• Molecular Weight: 93,886 Da
• NCBI Official Full Name: ATP-binding cassette sub-family B member 6, mitochondrial
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 6
• NCBI Official Symbol: ABCB6
• NCBI Official Synonym Symbols: ABC; PRP; umat; ABC14; MTABC3; FLJ22414
• NCBI Protein Information: ATP-binding cassette sub-family B member 6, mitochondrial; OTTHUMP00000164171; OTTHUMP00000206108; mt-ABC transporter 3; P-glycoprotein-related protein; mitochondrial ABC transporter 3; ATP-binding cassette half-transporter; ubiquitously-expressed mammali
• UniProt Protein Name: ATP-binding cassette sub-family B member 6, mitochondrial
• Protein Family: ABC transporter B family
• UniProt Gene Name: ABCB6
• UniProt Synonym Gene Names: MTABC3; PRP; UMAT; Mt-ABC transporter 3
• UniProt Entry Name: ABCB6_HUMAN

Uniprot Description

ABCB6: Binds heme and porphyrins and functions in their ATP- dependent uptake into the mitochondria. Plays a crucial role in heme synthesis. Defects in ABCB6 are the cause of microphthalmia, isolated, with coloboma, type 7 (MCOPCB7). A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, ABC family; Hydrolase; Mitochondrial; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: mitochondrial envelope; Golgi apparatus; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; plasma membrane; ATP-binding cassette (ABC) transporter complex; endosome; integral to mitochondrial outer membrane

Molecular Function: efflux transmembrane transporter activity; heme-transporting ATPase activity; heme transporter activity; heme binding; ATP binding

Biological Process: skin development; heme transport; cellular iron ion homeostasis; transport; brain development; porphyrin biosynthetic process; transmembrane transport

Disease: Dyschromatosis Universalis Hereditaria 3; Blood Group, Langereis System; Microphthalmia, Isolated, With Coloboma 7