Western Blot (WB)
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)
Rat anti-Mouse CD68 Monoclonal Antibody | anti-CD68 antibody
Rat anti Mouse CD68:Amethyst Orange
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Amethyst Orange - liquid
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Western Blot (WB)
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)
Western Blot (WB)
(Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)
Testing Data
(Frozen mouse lymph node stained with rat anti-mouse CD68 at a 1/100 dilution followed by goat anti-rat IgG:HRP at a 1/50 dilution.)
Testing Data
(Frozen mouse lymph node stained with rat anti-mouse CD68 at a 1/100 dilution followed by goat anti-rat IgG:HRP at a 1/50 dilution.)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody. High power)
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody. High power)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Immunofluorescence (IF)
(Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue u)
Dot Blot (DB)
(Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 at a 1/5 dilution and CD88 at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock (BUF041B). Permeabilisat)
Dot Blot (DB)
(Dot Blot showing FSC/SSC gated mouse peritoneal macrophages dual stained with CD68 at a 1/5 dilution and CD88 at a 1/5 dilution. Isotype control pair in red. Fc receptors were blocked by mouse Seroblock (BUF041B). Permeabilisat)
Testing Data
(Published customer image:Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.Image caption:CB2 receptors are expressed in microglial cells and do not accumulate in)
Testing Data
(Published customer image:Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.Image caption:CB2 receptors are expressed in microglial cells and do not accumulate in)
Testing Data
(Published customer image:Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.Image caption:Comparison of CB2 immunoreactivity in neurons, activated microglia and astrocytes. ()
Testing Data
(Published customer image:Rat anti Mouse CD68 antibody, clone FA-11 used for the identification of microglia in mouse brain by immunofluorescence.Image caption:Comparison of CB2 immunoreactivity in neurons, activated microglia and astrocytes. ()
CD68 is considered a pan macrophage marker, predominantly expressed on the intracellular lysosomes of tissue macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.
CD68 is expressed by many tumor types including some B cell lymphomas, blastic NK lymphomas, melanomas, granulocytic (myeloid) sarcomas, hairy cell leukemias, and renal, urinary and pancreatic tumors, and can be used in cancer studies to demonstrate the presence/localization of macrophages.
Rat anti mouse CD68 antibody, clone FA-11, has been used in many mouse models for the identification of CD68 in immunohistochemical studies, using both frozen and paraffin-embedded tissues (Masaki et al. 2003) and (Devey et al. 2009).
Rat anti mouse CD68 antibody, clone FA-11 can be used in flow cytometry to detect intracellular CD68, following permeabilization, and can detect surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
NCBI and Uniprot Product Information
Uniprot Description
CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Membrane protein, integral
Cellular Component: membrane; lysosome; integral to membrane; plasma membrane; endosome
Research Articles on CD68
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Product Notes
The CD68 cd68 (Catalog #AAA226123) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti Mouse CD68:Amethyst Orange reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD68 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Neat to 1/10. Researchers should empirically determine the suitability of the CD68 cd68 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD68, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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