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Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:RPE (MBS219491))
Mouse CD65s Monoclonal Antibody | anti-CD65s antibody
MOUSE ANTI HUMAN CD65s:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
CD65s; Monoclonal Antibody; MOUSE ANTI HUMAN CD65s:FITC; anti-CD65s antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
VIM-2
Form/Format
FITC
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Purified IgM conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid.
Concentration
IgM concentration 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD65s antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: neat; Maximum Dilution: 1/10
Flow Cytometry: Minimum Dilution: neat; Maximum Dilution: 1/10
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Immunogen
THP1 (human acute monocytic leukaemia cells)
Fusion Partners
Fusion Partners
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:RPE (MBS219491))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:RPE (MBS219491))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Low Endotoxin (MBS216219))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Low Endotoxin (MBS216219))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:FITC)
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:FITC)
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Alexa Fluor 647 (MBS217284A647))
Testing Data
(Staining of human peripheral blood granulocytes with Mouse anti Human CD65s:Alexa Fluor 647 (MBS217284A647))
Related Product Information for anti-CD65s antibody
Mouse anti Human CD65s antibody, clone VIM-2 selectively recognizes the sialylated form of human CD65, known as CD65s (VIM-2 antigen), a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage. CD65s is aberrantly expressed on some acute myeloid leukaemias (AML) and clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation (Lund-Johansen et al. 1992).
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Product Notes
The CD65s (Catalog #AAA217287) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD65s can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the CD65s for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD65s, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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